2pa8: Difference between revisions

Revision as of 23:07, 16 April 2008

X-Ray Crystal Structure of the D/L Subcomplex of the Sulfolobus Solfataricus RNA polymerase

OverviewOverview

The transcription apparatus in Archaea can be described as a simplified version of its eukaryotic RNA polymerase (RNAP) II counterpart, comprising an RNAPII-like enzyme as well as two general transcription factors, the TATA-binding protein (TBP) and the eukaryotic TFIIB orthologue TFB. It has been widely understood that precise comparisons of cellular RNAP crystal structures could reveal structural elements common to all enzymes and that these insights would be useful in analysing components of each enzyme that enable it to perform domain-specific gene expression. However, the structure of archaeal RNAP has been limited to individual subunits. Here we report the first crystal structure of the archaeal RNAP from Sulfolobus solfataricus at 3.4 A resolution, completing the suite of multi-subunit RNAP structures from all three domains of life. We also report the high-resolution (at 1.76 A) crystal structure of the D/L subcomplex of archaeal RNAP and provide the first experimental evidence of any RNAP possessing an iron-sulphur (Fe-S) cluster, which may play a structural role in a key subunit of RNAP assembly. The striking structural similarity between archaeal RNAP and eukaryotic RNAPII highlights the simpler archaeal RNAP as an ideal model system for dissecting the molecular basis of eukaryotic transcription.

About this StructureAbout this Structure

2PA8 is a Protein complex structure of sequences from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

ReferenceReference

The X-ray crystal structure of RNA polymerase from Archaea., Hirata A, Klein BJ, Murakami KS, Nature. 2008 Feb 14;451(7180):851-4. Epub 2008 Jan 30. PMID:18235446 Page seeded by OCA on Wed Apr 16 23:07:15 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:2pa8.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2pa8 |SIZE=350|CAPTION= <scene name='initialview01'>2pa8</scene>, resolution 1.760&Aring;
+The line below this paragraph, containing "STRUCTURE_2pa8", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+D+400'>AC1</scene> and <scene name='pdbsite=AC2:F3s+Binding+Site+For+Residue+D+3076'>AC2</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= rpoD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus]), rpoL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus])
+-->
-|DOMAIN=
+{{STRUCTURE_2pa8|  PDB=2pa8  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pa8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pa8 OCA], [http://www.ebi.ac.uk/pdbsum/2pa8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pa8 RCSB]</span>
-}}
 '''X-Ray Crystal Structure of the D/L Subcomplex of the Sulfolobus Solfataricus RNA polymerase'''
@@ Line 28: / Line 25: @@
 [[Category: Hirata, A.]]
 [[Category: Murakami, K S.]]
-[[Category: ferredoxin-like fe-s binding motif]]
+[[Category: Ferredoxin-like fe-s binding motif]]
-[[Category: platform for rna polymerase assembly]]
+[[Category: Platform for rna polymerase assembly]]
-[[Category: transferase]]
+[[Category: Transferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 16 23:07:15 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:33:28 2008''