6e63: Difference between revisions
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==Crystal structure of malaria transmission-blocking antigen Pfs48/45 6C in complex with antibody TB31F== | |||
<StructureSection load='6e63' size='340' side='right' caption='[[6e63]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6e63]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E63 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E63 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e63 OCA], [http://pdbe.org/6e63 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e63 RCSB], [http://www.ebi.ac.uk/pdbsum/6e63 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e63 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Interventions that can block the transmission of malaria-causing Plasmodium falciparum (Pf) between the human host and Anopheles vector have the potential to reduce the incidence of malaria. Pfs48/45 is a gametocyte surface protein critical for parasite development and transmission, and its targeting by monoclonal antibody (mAb) 85RF45.1 leads to the potent reduction of parasite transmission. Here, we reveal how the Pfs48/45 6C domain adopts a (SAG1)-related-sequence (SRS) fold. We structurally delineate potent epitope I and show how mAb 85RF45.1 recognizes an electronegative surface with nanomolar affinity. Analysis of Pfs48/45 sequences reveals that polymorphisms are rare for residues involved at the binding interface. Humanization of rat-derived mAb 85RF45.1 conserved the mode of recognition and activity of the parental antibody, while also improving its thermostability. Our work has implications for the development of transmission-blocking interventions, both through improving vaccine designs and the testing of passive delivery of mAbs in humans. | |||
Structural delineation of potent transmission-blocking epitope I on malaria antigen Pfs48/45.,Kundu P, Semesi A, Jore MM, Morin MJ, Price VL, Liang A, Li J, Miura K, Sauerwein RW, King CR, Julien JP Nat Commun. 2018 Oct 26;9(1):4458. doi: 10.1038/s41467-018-06742-9. PMID:30367064<ref>PMID:30367064</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6e63" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Human]] | |||
[[Category: Plafa]] | |||
[[Category: Julien, J P]] | |||
[[Category: Kundu, P]] | |||
[[Category: Semesi, A]] | [[Category: Semesi, A]] | ||
[[Category: | [[Category: Antibody]] | ||
[[Category: | [[Category: Antigen]] | ||
[[Category: Immune system]] | |||
[[Category: Malaria]] | |||
[[Category: Transmission-blocking]] | |||
Revision as of 23:28, 2 December 2018
Crystal structure of malaria transmission-blocking antigen Pfs48/45 6C in complex with antibody TB31FCrystal structure of malaria transmission-blocking antigen Pfs48/45 6C in complex with antibody TB31F
Structural highlights
Publication Abstract from PubMedInterventions that can block the transmission of malaria-causing Plasmodium falciparum (Pf) between the human host and Anopheles vector have the potential to reduce the incidence of malaria. Pfs48/45 is a gametocyte surface protein critical for parasite development and transmission, and its targeting by monoclonal antibody (mAb) 85RF45.1 leads to the potent reduction of parasite transmission. Here, we reveal how the Pfs48/45 6C domain adopts a (SAG1)-related-sequence (SRS) fold. We structurally delineate potent epitope I and show how mAb 85RF45.1 recognizes an electronegative surface with nanomolar affinity. Analysis of Pfs48/45 sequences reveals that polymorphisms are rare for residues involved at the binding interface. Humanization of rat-derived mAb 85RF45.1 conserved the mode of recognition and activity of the parental antibody, while also improving its thermostability. Our work has implications for the development of transmission-blocking interventions, both through improving vaccine designs and the testing of passive delivery of mAbs in humans. Structural delineation of potent transmission-blocking epitope I on malaria antigen Pfs48/45.,Kundu P, Semesi A, Jore MM, Morin MJ, Price VL, Liang A, Li J, Miura K, Sauerwein RW, King CR, Julien JP Nat Commun. 2018 Oct 26;9(1):4458. doi: 10.1038/s41467-018-06742-9. PMID:30367064[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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