2oz9: Difference between revisions
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'''E. coli TRP holorepressor, orthorhombic crystal form''' | '''E. coli TRP holorepressor, orthorhombic crystal form''' | ||
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==About this Structure== | ==About this Structure== | ||
2OZ9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry | 2OZ9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2wrp 2wrp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OZ9 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Lawson, C L.]] | [[Category: Lawson, C L.]] | ||
[[Category: Sigler, P B.]] | [[Category: Sigler, P B.]] | ||
[[Category: | [[Category: Dna binding regulatory protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:58:59 2008'' | |||
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Revision as of 11:59, 4 May 2008
E. coli TRP holorepressor, orthorhombic crystal form
OverviewOverview
An orthorhombic crystal form of trp repressor (aporepressor plus L-tryptophan ligand) was solved by molecular replacement, refined to 1.65 A resolution, and compared to the structure of the repressor in trigonal crystals. Even though these two crystal forms of repressor were grown under identical conditions, the refined structures have distinctly different conformations of the DNA-binding domains. Unlike the repressor/aporepressor structural transition, the conformational shift is not caused by the binding or loss of the L-tryptophan ligand. We conclude that while L-tryptophan binding is essential for forming a specific complex with trp operator DNA, the corepressor ligand does not lock the repressor into a single conformation that is complementary to the operator. This flexibility may be required by the various binding modes proposed for trp repressor in its search for and adherence to its three different operator sites.
About this StructureAbout this Structure
2OZ9 is a Single protein structure of sequence from Escherichia coli. This structure supersedes the now removed PDB entry 2wrp. Full crystallographic information is available from OCA.
ReferenceReference
Flexibility of the DNA-binding domains of trp repressor., Lawson CL, Zhang RG, Schevitz RW, Otwinowski Z, Joachimiak A, Sigler PB, Proteins. 1988;3(1):18-31. PMID:3375234 Page seeded by OCA on Sun May 4 11:58:59 2008