2oyt: Difference between revisions

Revision as of 11:57, 4 May 2008

Crystal Structure of UNG2/DNA(TM)

OverviewOverview

The enzyme uracil DNA glycosylase (UNG) excises unwanted uracil bases in the genome using an extrahelical base recognition mechanism. Efficient removal of uracil is essential for prevention of C-to-T transition mutations arising from cytosine deamination, cytotoxic U*A pairs arising from incorporation of dUTP in DNA, and for increasing immunoglobulin gene diversity during the acquired immune response. A central event in all of these UNG-mediated processes is the singling out of rare U*A or U*G base pairs in a background of approximately 10(9) T*A or C*G base pairs in the human genome. Here we establish for the human and Escherichia coli enzymes that discrimination of thymine and uracil is initiated by thermally induced opening of T*A and U*A base pairs and not by active participation of the enzyme. Thus, base-pair dynamics has a critical role in the genome-wide search for uracil, and may be involved in initial damage recognition by other DNA repair glycosylases.

About this StructureAbout this Structure

2OYT is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Enzymatic capture of an extrahelical thymine in the search for uracil in DNA., Parker JB, Bianchet MA, Krosky DJ, Friedman JI, Amzel LM, Stivers JT, Nature. 2007 Sep 27;449(7161):433-7. Epub 2007 Aug 19. PMID:17704764 Page seeded by OCA on Sun May 4 11:57:23 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2oyt.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2oyt |SIZE=350|CAPTION= <scene name='initialview01'>2oyt</scene>, resolution 2.000&Aring;
+The line below this paragraph, containing "STRUCTURE_2oyt", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=4MF:1-(2-DEOXY-5-O-PHOSPHONO-BETA-D-ERYTHRO-PENTOFURANOSYL)-4-METHYL-1H-INDOLE'>4MF</scene>, <scene name='pdbligand=AAB:2-DEOXY-5-PHOSPHORIBOSE+GROUP'>AAB</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= UNG, DGU, UNG1, UNG15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_2oyt|  PDB=2oyt  |  SCENE=  }}
-|RELATEDENTRY=[[2oxm|2OXM]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oyt OCA], [http://www.ebi.ac.uk/pdbsum/2oyt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oyt RCSB]</span>
-}}
 '''Crystal Structure of UNG2/DNA(TM)'''
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 [[Category: D J., Krosky.]]
 [[Category: Stivers, J T]]
-[[Category: enzyme-dna complex]]
+[[Category: Enzyme-dna complex]]
-[[Category: hydrolase/dna complex]]
+[[Category: Hydrolase/dna complex]]
-[[Category: ung2]]
+[[Category: Ung2]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 11:57:23 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:25:13 2008''