2otl: Difference between revisions

Revision as of 11:37, 4 May 2008

Girodazole bound to the large subunit of Haloarcula marismortui

OverviewOverview

Crystal structures of the 50 S ribosomal subunit from Haloarcula marismortui complexed with two antibiotics have identified new sites at which antibiotics interact with the ribosome and inhibit protein synthesis. 13-Deoxytedanolide binds to the E site of the 50 S subunit at the same location as the CCA of tRNA, and thus appears to inhibit protein synthesis by competing with deacylated tRNAs for E site binding. Girodazole binds near the E site region, but is somewhat buried and may inhibit tRNA binding by interfering with conformational changes that occur at the E site. The specificity of 13-deoxytedanolide for eukaryotic ribosomes is explained by its extensive interactions with protein L44e, which is an E site component of archaeal and eukaryotic ribosomes, but not of eubacterial ribosomes. In addition, protein L28, which is unique to the eubacterial E site, overlaps the site occupied by 13-deoxytedanolide, precluding its binding to eubacterial ribosomes. Girodazole is specific for eukarytes and archaea because it makes interactions with L15 that are not possible in eubacteria.

About this StructureAbout this Structure

2OTL is a Protein complex structure of sequences from Haloarcula marismortui. Full crystallographic information is available from OCA.

ReferenceReference

The structures of antibiotics bound to the E site region of the 50 S ribosomal subunit of Haloarcula marismortui: 13-deoxytedanolide and girodazole., Schroeder SJ, Blaha G, Tirado-Rives J, Steitz TA, Moore PB, J Mol Biol. 2007 Apr 13;367(5):1471-9. Epub 2007 Feb 7. PMID:17321546 Page seeded by OCA on Sun May 4 11:37:47 2008

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OCA

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 [[Image:2otl.jpg|left|200px]]
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-|RELATEDENTRY=[[2otj|2OTJ]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2otl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2otl OCA], [http://www.ebi.ac.uk/pdbsum/2otl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2otl RCSB]</span>
-}}
 '''Girodazole bound to the large subunit of Haloarcula marismortui'''
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 [[Category: Tirado-Rives, J.]]
 [[Category: 50]]
-[[Category: antibiotic complex]]
+[[Category: Antibiotic complex]]
-[[Category: girodazole]]
+[[Category: Girodazole]]
-[[Category: girolline]]
+[[Category: Girolline]]
-[[Category: large subunit]]
+[[Category: Large subunit]]
-[[Category: ribosome]]
+[[Category: Ribosome]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 11:37:47 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:22:55 2008''