2oqr: Difference between revisions

Revision as of 11:28, 4 May 2008

The structure of the response regulator RegX3 from Mycobacterium tuberculosis

OverviewOverview

The full-length, two-domain response regulator RegX3 from Mycobacterium tuberculosis is a dimer stabilized by three-dimensional domain swapping. Dimerization is known to occur in the OmpR/PhoB subfamily of response regulators upon activation but has previously only been structurally characterized for isolated receiver domains. The RegX3 dimer has a bipartite intermolecular interface, which buries 2357 A(2) per monomer. The two parts of the interface are between the two receiver domains (dimerization interface) and between a composite receiver domain and the effector domain of the second molecule (interdomain interface). The structure provides support for the importance of threonine and tyrosine residues in the signal transduction mechanism. These residues occur in an active-like conformation stabilized by lanthanum ions. In solution, RegX3 exists as both a monomer and a dimer in a concentration-dependent equilibrium. The dimer in solution differs from the active form observed in the crystal, resembling instead the model of the inactive full-length response regulator PhoB.

About this StructureAbout this Structure

2OQR is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

The structure of a full-length response regulator from Mycobacterium tuberculosis in a stabilized three-dimensional domain-swapped, activated state., King-Scott J, Nowak E, Mylonas E, Panjikar S, Roessle M, Svergun DI, Tucker PA, J Biol Chem. 2007 Dec 28;282(52):37717-29. Epub 2007 Oct 16. PMID:17942407 Page seeded by OCA on Sun May 4 11:28:37 2008

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OCA

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 [[Image:2oqr.gif|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_2oqr", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene>
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-|ACTIVITY=
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-|GENE= regX3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
+-->
-|DOMAIN=
+{{STRUCTURE_2oqr|  PDB=2oqr  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oqr OCA], [http://www.ebi.ac.uk/pdbsum/2oqr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oqr RCSB]</span>
-}}
 '''The structure of the response regulator RegX3 from Mycobacterium tuberculosis'''
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 [[Category: King-Scott, J.]]
 [[Category: 3d domain swapping]]
-[[Category: dna-binding]]
+[[Category: Dna-binding]]
-[[Category: regx3]]
+[[Category: Regx3]]
-[[Category: response regulator]]
+[[Category: Response regulator]]
-[[Category: transcription,signaling protein]]
+[[Category: Transcription,signaling protein]]
-[[Category: two component system]]
+[[Category: Two component system]]
-[[Category: winged-helix-turn-helix]]
+[[Category: Winged-helix-turn-helix]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 11:28:37 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:21:47 2008''