2bj9: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2bj9.gif|left|200px]]<br /> | [[Image:2bj9.gif|left|200px]]<br /><applet load="2bj9" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2bj9" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="2bj9, resolution 3.00Å" /> | caption="2bj9, resolution 3.00Å" /> | ||
'''NIKR WITH BOUND NICKEL AND PHOSPHATE'''<br /> | '''NIKR WITH BOUND NICKEL AND PHOSPHATE'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
2BJ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with NI, PO4 and PG4 as [http://en.wikipedia.org/wiki/ligands ligands]. | 2BJ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with NI, PO4 and PG4 as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Pg4 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BJ9 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 32: | Line 31: | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:49:10 2007'' | ||
Revision as of 19:39, 18 December 2007
|
NIKR WITH BOUND NICKEL AND PHOSPHATE
OverviewOverview
The Pyrococcus horikoshii OT3 genome contains a gene (PH0601 or nikR), encoding a protein (PhNikR) that shares 33.8% amino acid sequence identity, with Escherichia coli nickel responsive repressor NikR (EcNikR), including, many residues that are functionally important in the E.coli ortholog. We, succeeded in crystallization and structural characterization of PhNikR in, the apo form and two nickel bound forms that exhibit different, conformations, open and closed. Moreover, we have identified a putative, "low-affinity" nickel-binding pocket in the closed form. This binding site, has unusual nickel coordination and exhibits high sensitivity to phosphate, in the crystal structure. Analysis of the PhNikR structures and, structure-based mutational studies with EcNikR reveals a plausible, mechanism of nickel-dependent promoter recognition by the NikR family of, proteins.
About this StructureAbout this Structure
2BJ9 is a Single protein structure of sequence from Pyrococcus horikoshii with NI, PO4 and PG4 as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structure of Pyrococcus horikoshii NikR: nickel sensing and implications for the regulation of DNA recognition., Chivers PT, Tahirov TH, J Mol Biol. 2005 May 6;348(3):597-607. PMID:15826657
Page seeded by OCA on Tue Dec 18 18:49:10 2007
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Pyrococcus horikoshii
- Single protein
- Chivers, P.T.
- RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.
- Tahirov, T.H.
- NI
- PG4
- PO4
- Dna-binding
- Metal-binding
- Nickel
- Nikr
- Repressor
- Riken structural genomics/proteomics initiative
- Rsgi
- Structural genomics
- Transcription
- Transcription regulation