2ohc: Difference between revisions

Revision as of 10:55, 4 May 2008

structural and mutational analysis of tRNA-intron splicing endonuclease from Thermoplasma acidophilum DSM1728

OverviewOverview

In archaea, RNA endonucleases that act specifically on RNA with bulge-helix-bulge motifs play the main role in the recognition and excision of introns, while the eukaryal enzymes use a measuring mechanism to determine the positions of the universally positioned splice sites relative to the conserved domain of pre-tRNA. Two crystallographic structures of tRNA intron-splicing endonuclease from Thermoplasma acidophilum DSM 1728 (EndA(Ta)) have been solved to 2.5-A and 2.7-A resolution by molecular replacement, using the 2.7-A resolution data as the initial model and the single-wavelength anomalous-dispersion phasing method using selenomethionine as anomalous signals, respectively. The models show that EndA(Ta) is a homodimer and that it has overall folding similar to that of other archaeal tRNA endonucleases. From structural and mutational analyses of H236A, Y229F, and K265I in vitro, we have demonstrated that they play critical roles in recognizing the splice site and in cleaving the pre-tRNA substrate.

About this StructureAbout this Structure

2OHC is a Single protein structure of sequence from Thermoplasma acidophilum. Full crystallographic information is available from OCA.

ReferenceReference

Structural and mutational analysis of tRNA intron-splicing endonuclease from Thermoplasma acidophilum DSM 1728: catalytic mechanism of tRNA intron-splicing endonucleases., Kim YK, Mizutani K, Rhee KH, Nam KH, Lee WH, Lee EH, Kim EE, Park SY, Hwang KY, J Bacteriol. 2007 Nov;189(22):8339-46. Epub 2007 Sep 7. PMID:17827289 Page seeded by OCA on Sun May 4 10:55:28 2008

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OCA

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 [[Image:2ohc.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2ohc |SIZE=350|CAPTION= <scene name='initialview01'>2ohc</scene>, resolution 2.50&Aring;
+The line below this paragraph, containing "STRUCTURE_2ohc", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= endA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2303 Thermoplasma acidophilum])
+-->
-|DOMAIN=
+{{STRUCTURE_2ohc|  PDB=2ohc  |  SCENE=  }}
-|RELATEDENTRY=[[2ohe|2OHE]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ohc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ohc OCA], [http://www.ebi.ac.uk/pdbsum/2ohc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ohc RCSB]</span>
-}}
 '''structural and mutational analysis of tRNA-intron splicing endonuclease from Thermoplasma acidophilum DSM1728'''
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 [[Category: Single protein]]
 [[Category: Thermoplasma acidophilum]]
-[[Category: tRNA-intron endonuclease]]
+[[Category: TRNA-intron endonuclease]]
 [[Category: Hwang, K Y.]]
 [[Category: Kim, Y K.]]
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 [[Category: Park, S Y.]]
 [[Category: Rhee, K H.]]
-[[Category: endonuclease]]
+[[Category: Endonuclease]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: intron]]
+[[Category: Intron]]
-[[Category: splicing]]
+[[Category: Splicing]]
-[[Category: trna]]
+[[Category: Trna]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 10:55:28 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:17:51 2008''