2eh2: Difference between revisions

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==Mutant V18M structure of PH0725 from Pyrococcus horikoshii OT3==
==Mutant V18M structure of PH0725 from Pyrococcus horikoshii OT3==
<StructureSection load='2eh2' size='340' side='right' caption='[[2eh2]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='2eh2' size='340' side='right'caption='[[2eh2]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2eh2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EH2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EH2 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2eh2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EH2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2EH2 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diphthine_synthase Diphthine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.98 2.1.1.98] </span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diphthine_synthase Diphthine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.98 2.1.1.98] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eh2 OCA], [http://pdbe.org/2eh2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eh2 RCSB], [http://www.ebi.ac.uk/pdbsum/2eh2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2eh2 ProSAT], [http://www.topsan.org/Proteins/RSGI/2eh2 TOPSAN]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2eh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eh2 OCA], [http://pdbe.org/2eh2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eh2 RCSB], [http://www.ebi.ac.uk/pdbsum/2eh2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2eh2 ProSAT], [http://www.topsan.org/Proteins/RSGI/2eh2 TOPSAN]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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</StructureSection>
</StructureSection>
[[Category: Diphthine synthase]]
[[Category: Diphthine synthase]]
[[Category: Large Structures]]
[[Category: Pyrococcus horikoshii]]
[[Category: Pyrococcus horikoshii]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]

Revision as of 10:31, 29 April 2020

Mutant V18M structure of PH0725 from Pyrococcus horikoshii OT3Mutant V18M structure of PH0725 from Pyrococcus horikoshii OT3

Structural highlights

2eh2 is a 2 chain structure with sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, ,
Activity:Diphthine synthase, with EC number 2.1.1.98
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

[DPHB_PYRHO] S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Zhu X, Kim J, Su X, Lin H. Reconstitution of diphthine synthase activity in vitro. Biochemistry. 2010 Nov 9;49(44):9649-57. doi: 10.1021/bi100812h. PMID:20873788 doi:http://dx.doi.org/10.1021/bi100812h

2eh2, resolution 2.00Å

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