2o7l: Difference between revisions
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'''The open-cap conformation of GlpG''' | '''The open-cap conformation of GlpG''' | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ha, Y.]] | [[Category: Ha, Y.]] | ||
[[Category: | [[Category: Glpg]] | ||
[[Category: | [[Category: Intramembrane proteolysis]] | ||
[[Category: | [[Category: Membrane protein]] | ||
[[Category: | [[Category: Rhomboid protease]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:25:18 2008'' | |||
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Revision as of 10:25, 4 May 2008
The open-cap conformation of GlpG
OverviewOverview
The active sites of intramembrane proteases are positioned in the lipid bilayer to facilitate peptide bond hydrolysis in the membrane. Previous crystallographic analysis of Escherichia coli GlpG, an intramembrane protease of the rhomboid family, has revealed an internal and hydrophilic active site in an apparently closed conformation. Here we describe the crystal structure of GlpG in a more open conformation, where the capping loop L5 has been lifted, exposing the previously buried and catalytically essential Ser-201 to outside aqueous solution. A water molecule now moves into the putative oxyanion hole that is constituted of a main-chain amide (Ser-201) and two conserved side chains (His-150 and Asn-154). The loop movement also destabilizes a hydrophobic side chain (Phe-245) previously buried between transmembrane helices S2 and S5 and creates a side portal from the lipid to protease active site. These results provide insights into the conformational plasticity of GlpG to accommodate substrate binding and catalysis and into the chirality of the reaction intermediate.
About this StructureAbout this Structure
2O7L is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Open-cap conformation of intramembrane protease GlpG., Wang Y, Ha Y, Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2098-102. Epub 2007 Feb 2. PMID:17277078 Page seeded by OCA on Sun May 4 10:25:18 2008