6dgc: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of the C-terminal catalytic domain of ISC1926 TnpA, an IS607-like serine recombinase== | ==Crystal structure of the C-terminal catalytic domain of ISC1926 TnpA, an IS607-like serine recombinase== | ||
<StructureSection load='6dgc' size='340' side='right' caption='[[6dgc]], [[Resolution|resolution]] 2.92Å' scene=''> | <StructureSection load='6dgc' size='340' side='right'caption='[[6dgc]], [[Resolution|resolution]] 2.92Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6dgc]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DGC FirstGlance]. <br> | <table><tr><td colspan='2'>[[6dgc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_sp._l00_11 Sulfolobus sp. l00 11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DGC FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgc OCA], [http://pdbe.org/6dgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dgc RCSB], [http://www.ebi.ac.uk/pdbsum/6dgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgc ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgc OCA], [http://pdbe.org/6dgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dgc RCSB], [http://www.ebi.ac.uk/pdbsum/6dgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
IS607-family transposons are unusual because they do not have terminal inverted repeats or generate target site duplications. They encode two protein-coding genes, but only tnpA is required for transposition. Our X-ray structures confirm that TnpA is a member of the serine recombinase (SR) family, but the chemically-inactive quaternary structure of the dimer, along with the N-terminal location of the DNA binding domain, are different from other SRs. TnpA dimers from IS1535 cooperatively associate with multiple subterminal repeats, which together with additional nonspecific binding, form a nucleoprotein filament on one transposon end that efficiently captures a second unbound end to generate the paired-end complex (PEC). Formation of the PEC does not require a change in the dimeric structure of the catalytic domain, but remodeling of the C-terminal alpha-helical region is involved. We posit that the PEC recruits a chemically-active conformer of TnpA to the transposon end to initiate DNA chemistry. | |||
Multiple serine transposase dimers assemble the transposon-end synaptic complex during IS607-family transposition.,Chen W, Mandali S, Hancock SP, Kumar P, Collazo M, Cascio D, Johnson RC Elife. 2018 Oct 5;7. pii: 39611. doi: 10.7554/eLife.39611. PMID:30289389<ref>PMID:30289389</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6dgc" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Sulfolobus sp. l00 11]] | |||
[[Category: Cascio, D]] | [[Category: Cascio, D]] | ||
[[Category: Hancock, S P]] | [[Category: Hancock, S P]] | ||