2nug: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2nug.gif|left|200px]] | [[Image:2nug.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_2nug", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_2nug| PDB=2nug | SCENE= }} | |||
}} | |||
'''Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA at 1.7-Angstrom Resolution''' | '''Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA at 1.7-Angstrom Resolution''' | ||
| Line 32: | Line 29: | ||
[[Category: Tropea, J E.]] | [[Category: Tropea, J E.]] | ||
[[Category: Waugh, D S.]] | [[Category: Waugh, D S.]] | ||
[[Category: | [[Category: Dsrna]] | ||
[[Category: | [[Category: Endonucleolytic cleavage]] | ||
[[Category: | [[Category: Hydrolase/rna complex]] | ||
[[Category: | [[Category: Ribonuclease iii]] | ||
[[Category: | [[Category: Rna interference]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:55:32 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 09:55, 4 May 2008
Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA at 1.7-Angstrom Resolution
OverviewOverview
RNA interference is mediated by small interfering RNAs produced by members of the ribonuclease III (RNase III) family represented by bacterial RNase III and eukaryotic Rnt1p, Drosha and Dicer. For mechanistic studies, bacterial RNase III has been a valuable model system for the family. Previously, we have shown that RNase III uses two catalytic sites to create the 2-nucleotide (nt) 3' overhangs in its products. Here, we present three crystal structures of RNase III in complex with double-stranded RNA, demonstrating how Mg(2+) is essential for the formation of a catalytically competent protein-RNA complex, how the use of two Mg(2+) ions can drive the hydrolysis of each phosphodiester bond, and how conformational changes in both the substrate and the protein are critical elements for assembling the catalytic complex. Moreover, we have modelled a protein-substrate complex and a protein-reaction intermediate (transition state) complex on the basis of the crystal structures. Together, the crystal structures and the models suggest a stepwise mechanism for RNase III to execute the phosphoryl transfer reaction.
About this StructureAbout this Structure
2NUG is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
ReferenceReference
A stepwise model for double-stranded RNA processing by ribonuclease III., Gan J, Shaw G, Tropea JE, Waugh DS, Court DL, Ji X, Mol Microbiol. 2007 Dec 7;67(1):143-154. Epub 2007 Nov 27. PMID:18047582 Page seeded by OCA on Sun May 4 09:55:32 2008