2jdf: Difference between revisions

Revision as of 08:44, 4 May 2008

HUMAN GAMMA-B CRYSTALLIN

OverviewOverview

The concept of novel binding proteins as an alternative to antibodies has undergone rapid development and is now ready for practical use in a wide range of applications. Alternative binding proteins, based on suitable scaffolds with desirable properties, are selected from combinatorial libraries in vitro. Here, we describe an approach using a beta-sheet of human gamma-B-crystallin to generate a universal binding site through randomization of eight solvent-exposed amino acid residues selected according to structural and sequence analyses. Specific variants, so-called Affilin, have been isolated from a phage display library against a variety of targets that differ considerably in size and structure. The isolated Affilin variants can be produced in Escherichia coli as soluble proteins and have a high level of thermodynamic stability. The crystal structures of the human wild-type gamma-B-crystallin and a selected Affilin variant have been determined to 1.7 A and 2.0 A resolution, respectively. Comparison of the two molecules indicates that the human gamma-B-crystallin tolerates amino acid exchanges with no major structural change. We conclude that the intrinsically stable and easily expressed gamma-B-crystallin provides a suitable framework for the generation of novel binding molecules.

About this StructureAbout this Structure

2JDF is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Affilin-novel binding molecules based on human gamma-B-crystallin, an all beta-sheet protein., Ebersbach H, Fiedler E, Scheuermann T, Fiedler M, Stubbs MT, Reimann C, Proetzel G, Rudolph R, Fiedler U, J Mol Biol. 2007 Sep 7;372(1):172-85. Epub 2007 Jun 22. PMID:17628592 Page seeded by OCA on Sun May 4 08:44:04 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2jdf.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2jdf |SIZE=350|CAPTION= <scene name='initialview01'>2jdf</scene>, resolution 1.7&Aring;
+The line below this paragraph, containing "STRUCTURE_2jdf", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2jdf|  PDB=2jdf  |  SCENE=  }}
-|RELATEDENTRY=[[1leu|1LEU]], [[1myv|1MYV]], [[1myx|1MYX]], [[1myy|1MYY]], [[1mz1|1MZ1]], [[1mz2|1MZ2]], [[1mz3|1MZ3]], [[2jdg|2JDG]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jdf OCA], [http://www.ebi.ac.uk/pdbsum/2jdf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jdf RCSB]</span>
-}}
 '''HUMAN GAMMA-B CRYSTALLIN'''
@@ Line 34: / Line 31: @@
 [[Category: Scheuermann, T.]]
 [[Category: Stubbs, M T.]]
-[[Category: affilin]]
+[[Category: Affilin]]
-[[Category: artificial binding protein]]
+[[Category: Artificial binding protein]]
-[[Category: eye lens protein]]
+[[Category: Eye lens protein]]
-[[Category: gamma crystallin]]
+[[Category: Gamma crystallin]]
-[[Category: oxidation]]
+[[Category: Oxidation]]
-[[Category: phosphorylation]]
+[[Category: Phosphorylation]]
-[[Category: polymorphism]]
+[[Category: Polymorphism]]
-[[Category: structural protein]]
+[[Category: Structural protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 08:44:04 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:56:33 2008''