2j71: Difference between revisions

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 [[Image:2j71.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2j71 |SIZE=350|CAPTION= <scene name='initialview01'>2j71</scene>, resolution 1.69&Aring;
+The line below this paragraph, containing "STRUCTURE_2j71", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pullulanase Pullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.41 3.2.1.41] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2j71|  PDB=2j71  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j71 OCA], [http://www.ebi.ac.uk/pdbsum/2j71 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2j71 RCSB]</span>
-}}
 '''ALPHA-GLUCAN RECOGNITION BY A FAMILY 41 CARBOHYDRATE-BINDING MODULE FROM THERMOTOGA MARITIMA PULLULANASE PULA'''
+==Overview==
+Starch recognition by carbohydrate-binding modules (CBMs) is important for the activity of starch-degrading enzymes. The N-terminal family 41 CBM, TmCBM41 (from pullulanase PulA secreted by Thermotoga maritima) was shown to have alpha-glucan binding activity with specificity for alpha-1,4-glucans but was able to tolerate the alpha-1,6-linkages found roughly every three or four glucose units in pullulan. Using X-ray crystallography, the structures were solved for TmCBM41 in an uncomplexed form and in complex with maltotetraose and 6(3)-alpha-D-glucosyl-maltotriose (GM3). Ligand binding was facilitated by stacking interactions between the alpha-faces of the glucose residues and two tryptophan side-chains in the two main subsites of the carbohydrate-binding site. Overall, this mode of starch binding is quite well conserved by other starch-binding modules. The structure in complex with GM3 revealed a third binding subsite with the flexibility to accommodate an alpha-1,4- or an alpha-1,6-linked glucose.
 ==About this Structure==
 J71 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J71 OCA].
+==Reference==
+The structural basis of alpha-glucan recognition by a family 41 carbohydrate-binding module from Thermotoga maritima., van Bueren AL, Boraston AB, J Mol Biol. 2007 Jan 19;365(3):555-60. Epub 2006 Oct 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17095014 17095014]
 [[Category: Pullulanase]]
 [[Category: Single protein]]
@@ Line 22: / Line 25: @@
 [[Category: Boraston, A B.]]
 [[Category: Bueren, A Lammerts Van.]]
-[[Category: alpha-glucan binding]]
+[[Category: Alpha-glucan binding]]
-[[Category: beta-sandwich fold]]
+[[Category: Beta-sandwich fold]]
-[[Category: carbohydrate-binding module]]
+[[Category: Carbohydrate-binding module]]
-[[Category: glycosidase]]
+[[Category: Glycosidase]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:26:32 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:53:55 2008''