2j4r: Difference between revisions

Revision as of 08:19, 4 May 2008

STRUCTURAL STUDY OF THE AQUIFEX AEOLICUS PPX-GPPA ENZYME

OverviewOverview

The crystal structure of the prototype exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family member from Aquifex aeolicus in complex with the intracellular second messenger guanosine tetraphosphate was determined at 2.7-A resolution. The hydrolytic base is identified as E119. The dual specificity established for the Escherichia coli homolog is shown to be compatible with a common active site for guanosine pentaphosphate and polyphosphate hydrolysis. Distinct and different degrees of closure between the two domains of the enzyme are associated with substrate binding. The arginines R22 and R267, residing in different domains, are crucial for guanosine pentaphosphate specificity as they interact with the unique 3'-ribose phosphorylation.

About this StructureAbout this Structure

2J4R is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the PPX/GPPA phosphatase from Aquifex aeolicus in complex with the alarmone ppGpp., Kristensen O, Ross B, Gajhede M, J Mol Biol. 2008 Feb 1;375(5):1469-76. Epub 2007 Dec 4. PMID:18155044 Page seeded by OCA on Sun May 4 08:19:58 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2j4r.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2j4r |SIZE=350|CAPTION= <scene name='initialview01'>2j4r</scene>, resolution 2.71&Aring;
+The line below this paragraph, containing "STRUCTURE_2j4r", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:G4p+Binding+Site+For+Chain+A'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=G4P:GUANOSINE-5&#39;,3&#39;-TETRAPHOSPHATE'>G4P</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2j4r|  PDB=2j4r  |  SCENE=  }}
-|RELATEDENTRY=[[1t6c|1T6C]], [[1t6d|1T6D]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j4r OCA], [http://www.ebi.ac.uk/pdbsum/2j4r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2j4r RCSB]</span>
-}}
 '''STRUCTURAL STUDY OF THE AQUIFEX AEOLICUS PPX-GPPA ENZYME'''
@@ Line 26: / Line 23: @@
 [[Category: Single protein]]
 [[Category: Kristensen, O.]]
-[[Category: aquifex aeolicus]]
+[[Category: Aquifex aeolicus]]
-[[Category: exopolyphosphatase]]
+[[Category: Exopolyphosphatase]]
-[[Category: guanosine pentaphosphate]]
+[[Category: Guanosine pentaphosphate]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: ppgpp]]
+[[Category: Ppgpp]]
-[[Category: stringent response]]
+[[Category: Stringent response]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 08:19:58 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:52:55 2008''