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The | ==Lariat-capping ribozyme with a shortened DP2 stem loop== | ||
<StructureSection load='6g7z' size='340' side='right' caption='[[6g7z]], [[Resolution|resolution]] 3.34Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6g7z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G7Z FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4p8z|4p8z]], [[4p95|4p95]], [[4p9r|4p9r]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g7z OCA], [http://pdbe.org/6g7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g7z RCSB], [http://www.ebi.ac.uk/pdbsum/6g7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g7z ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The lariat-capping (LC) ribozyme is a natural ribozyme isolated from eukaryotic microorganisms. Despite apparent structural similarity to group I introns, the LC ribozyme catalyzes cleavage by a 2',5' branching reaction, leaving the 3' product with a 3-nt lariat cap that functionally substitutes for a conventional mRNA cap in the downstream pre-mRNA encoding a homing endonuclease. We describe the crystal structures of the precleavage and postcleavage LC ribozymes, which suggest that structural features inherited from group I ribozymes have undergone speciation due to profound changes in molecular selection pressure, ultimately giving rise to an original branching ribozyme family. The structures elucidate the role of key elements that regulate the activity of the LC ribozyme by conformational switching and suggest a mechanism by which the signal for branching is transmitted to the catalytic core. The structures also show how conserved interactions twist residues, forming the lariat to join chemical groups involved in branching. | |||
Speciation of a group I intron into a lariat capping ribozyme.,Meyer M, Nielsen H, Olieric V, Roblin P, Johansen SD, Westhof E, Masquida B Proc Natl Acad Sci U S A. 2014 May 12. PMID:24821772<ref>PMID:24821772</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6g7z" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Masquida, B]] | |||
[[Category: Meyer, M]] | |||
[[Category: Olieric, V]] | [[Category: Olieric, V]] | ||
[[Category: | [[Category: Lariat capping ribozyme]] | ||
[[Category: | [[Category: Rna]] | ||