2ihe: Difference between revisions

Revision as of 07:30, 4 May 2008

Crystal structure of wild-type single-stranded DNA binding protein from Thermus aquaticus

OverviewOverview

In contrast to the majority of tetrameric SSB proteins, the recently discovered SSB proteins from the Thermus/Deinoccus group form dimers. We solved the crystal structures of the SSB protein from Thermus aquaticus (TaqSSB) and a deletion mutant of the protein and show the structure of their ssDNA binding domains to be similar to the structure of tetrameric SSBs. Two conformations accompanied by proline cis-trans isomerization are observed in the flexible C-terminal region. For the first time, we were able to trace 6 out of 10 amino acids at the C-terminus of an SSB protein. This highly conserved region is essential for interaction with other proteins and we show it to adopt an extended conformation devoid of secondary structure. A model for binding this region to the chi subunit of DNA polymerase III is proposed. It explains at a molecular level the reason for the ssb113 phenotype observed in Escherichia coli.

About this StructureAbout this Structure

2IHE is a Single protein structure of sequence from Thermus aquaticus. Full crystallographic information is available from OCA.

ReferenceReference

3D structure of Thermus aquaticus single-stranded DNA-binding protein gives insight into the functioning of SSB proteins., Fedorov R, Witte G, Urbanke C, Manstein DJ, Curth U, Nucleic Acids Res. 2006;34(22):6708-17. Epub 2006 Dec 5. PMID:17148487 Page seeded by OCA on Sun May 4 07:30:21 2008

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OCA

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 [[Image:2ihe.jpg|left|200px]]
- {{Structure
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-|GENE= ssb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus])
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-|RELATEDENTRY=[[1eyg|1EYG]], [[1se8|1SE8]], [[2ihf|2IHF]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ihe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ihe OCA], [http://www.ebi.ac.uk/pdbsum/2ihe PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ihe RCSB]</span>
-}}
 '''Crystal structure of wild-type single-stranded DNA binding protein from Thermus aquaticus'''
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 [[Category: Urbanke, C.]]
 [[Category: Witte, G.]]
-[[Category: protein-dna interaction]]
+[[Category: Protein-dna interaction]]
-[[Category: protein-protein interaction]]
+[[Category: Protein-protein interaction]]
-[[Category: single-stranded dna binding protein (ssb)]]
+[[Category: Thermophile organism]]
-[[Category: thermophile organism]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 07:30:21 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:44:22 2008''