2b2e: Difference between revisions
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==RNA stemloop from bacteriophage MS2 complexed with an N87S,E89K mutant MS2 capsid== | ==RNA stemloop from bacteriophage MS2 complexed with an N87S,E89K mutant MS2 capsid== | ||
<StructureSection load='2b2e' size='340' side='right' caption='[[2b2e]], [[Resolution|resolution]] 3.15Å' scene=''> | <StructureSection load='2b2e' size='340' side='right'caption='[[2b2e]], [[Resolution|resolution]] 3.15Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2b2e]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2E FirstGlance]. <br> | <table><tr><td colspan='2'>[[2b2e]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2E FirstGlance]. <br> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Bacteriophage ms2]] | [[Category: Bacteriophage ms2]] | ||
[[Category: Large Structures]] | |||
[[Category: Adams, C J]] | [[Category: Adams, C J]] | ||
[[Category: Baron, A J]] | [[Category: Baron, A J]] | ||
Revision as of 11:08, 19 February 2020
RNA stemloop from bacteriophage MS2 complexed with an N87S,E89K mutant MS2 capsidRNA stemloop from bacteriophage MS2 complexed with an N87S,E89K mutant MS2 capsid
Structural highlights
Function[COAT_BPMS2] Forms the phage shell; binds to the phage RNA. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedSequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism. Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2.,Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG Structure. 2006 Mar;14(3):487-95. PMID:16531233[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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