2i4a: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2i4a.gif|left|200px]] | [[Image:2i4a.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_2i4a", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_2i4a| PDB=2i4a | SCENE= }} | |||
}} | |||
'''Crystal structure of thioredoxin from the acidophile Acetobacter aceti''' | '''Crystal structure of thioredoxin from the acidophile Acetobacter aceti''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I4A OCA]. | |||
==Reference== | ==Reference== | ||
Atomic-resolution crystal structure of thioredoxin from the acidophilic bacterium Acetobacter aceti., Starks CM, Francois JA, MacArthur KM, Heard BZ, Kappock TJ, Protein Sci. 2007 Jan;16(1):92-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17192591 17192591] | Atomic-resolution crystal structure of thioredoxin from the acidophilic bacterium Acetobacter aceti., Starks CM, Francois JA, MacArthur KM, Heard BZ, Kappock TJ, Protein Sci. 2007 Jan;16(1):92-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17192591 17192591] | ||
[[Category: Kappock, T J.]] | [[Category: Kappock, T J.]] | ||
[[Category: Starks, C M.]] | [[Category: Starks, C M.]] | ||
[[Category: | [[Category: Acidophile]] | ||
[[Category: | [[Category: Disulfide exchange]] | ||
[[Category: | [[Category: Thioredoxin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:03:03 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 07:03, 4 May 2008
Crystal structure of thioredoxin from the acidophile Acetobacter aceti
OverviewOverview
The crystal structure of thioredoxin (AaTrx) from the acetic acid bacterium Acetobacter aceti was determined at 1 A resolution. This is currently the highest resolution crystal structure available for any thioredoxin. Thioredoxins facilitate thiol-disulfide exchange, a process that is expected to be slow at the low pH values encountered in the A. aceti cytoplasm. Despite the apparent need to function at low pH, neither the active site nor the surface charge distribution of AaTrx is notably different from that of Escherichia coli thioredoxin. Apparently the ancestral thioredoxin was sufficiently stable for use in A. aceti or the need to interact with multiple targets constrained the variation of surface residues. The AaTrx structure presented here provides a clear view of all ionizable protein moieties and waters, a first step in understanding how thiol-disulfide exchange might occur in a low pH cytoplasm, and is a basis for biophysical studies of the mechanism of acid-mediated unfolding. The high resolution of this structure should be useful for computational studies of thioredoxin function, protein structure and dynamics, and side-chain ionization.
About this StructureAbout this Structure
Full crystallographic information is available from OCA.
ReferenceReference
Atomic-resolution crystal structure of thioredoxin from the acidophilic bacterium Acetobacter aceti., Starks CM, Francois JA, MacArthur KM, Heard BZ, Kappock TJ, Protein Sci. 2007 Jan;16(1):92-8. PMID:17192591 Page seeded by OCA on Sun May 4 07:03:03 2008