2i3q: Difference between revisions

Revision as of 07:01, 4 May 2008

Q44V mutant of Homing Endonuclease I-CreI

OverviewOverview

Homing endonucleases are highly specific enzymes, capable of recognizing and cleaving unique DNA sequences in complex genomes. Since such DNA cleavage events can result in targeted allele-inactivation and/or allele-replacement in vivo, the ability to engineer homing endonucleases matched to specific DNA sequences of interest would enable powerful and precise genome manipulations. We have taken a step-wise genetic approach in analyzing individual homing endonuclease I-CreI protein/DNA contacts, and describe here novel interactions at four distinct target site positions. Crystal structures of two mutant endonucleases reveal the molecular interactions responsible for their altered DNA target specificities. We also combine novel contacts to create an endonuclease with the predicted target specificity. These studies provide important insights into engineering homing endonucleases with novel target specificities, as well as into the evolution of DNA recognition by this fascinating family of proteins.

About this StructureAbout this Structure

2I3Q is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

ReferenceReference

Homing endonuclease I-CreI derivatives with novel DNA target specificities., Rosen LE, Morrison HA, Masri S, Brown MJ, Springstubb B, Sussman D, Stoddard BL, Seligman LM, Nucleic Acids Res. 2006;34(17):4791-800. Epub 2006 Sep 13. PMID:16971456 Page seeded by OCA on Sun May 4 07:01:53 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2i3q.gif|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i3q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i3q OCA], [http://www.ebi.ac.uk/pdbsum/2i3q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i3q RCSB]</span>
-}}
 '''Q44V mutant of Homing Endonuclease I-CreI'''
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 [[Category: Rosen, L.]]
 [[Category: Sussman, D.]]
-[[Category: homing endonuclease]]
+[[Category: Homing endonuclease]]
-[[Category: i-crei]]
+[[Category: I-crei]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 07:01:53 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:39:13 2008''