2i1k: Difference between revisions

Revision as of 06:57, 4 May 2008

Moesin from Spodoptera frugiperda reveals the coiled-coil domain at 3.0 angstrom resolution

OverviewOverview

Ezrin/radixin/moesin (ERM) family members provide a regulated link between the cortical actin cytoskeleton and the plasma membrane to govern membrane structure and organization. Here, we report the crystal structure of intact insect moesin, revealing that its essential yet previously uncharacterized alpha-helical domain forms extensive interactions with conserved surfaces of the band four-point-one/ezrin/radixin/moesin (FERM) domain. These interdomain contacts provide a functional explanation for how PIP(2) binding and tyrosine phosphorylation of ezrin lead to activation, and provide an understanding of previously enigmatic loss-of-function missense mutations in the tumor suppressor merlin. Sequence conservation and biochemical results indicate that this structure represents a complete model for the closed state of all ERM-merlin proteins, wherein the central alpha-helical domain is an active participant in an extensive set of inhibitory interactions that can be unmasked, in a rheostat-like manner, by coincident regulatory factors that help determine cell polarity and membrane structure.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Self-masking in an intact ERM-merlin protein: an active role for the central alpha-helical domain., Li Q, Nance MR, Kulikauskas R, Nyberg K, Fehon R, Karplus PA, Bretscher A, Tesmer JJ, J Mol Biol. 2007 Feb 2;365(5):1446-59. Epub 2006 Oct 26. PMID:17134719 Page seeded by OCA on Sun May 4 06:57:56 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2i1k.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2i1k |SIZE=350|CAPTION= <scene name='initialview01'>2i1k</scene>, resolution 3.000&Aring;
+The line below this paragraph, containing "STRUCTURE_2i1k", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=URE:UREA'>URE</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2i1k|  PDB=2i1k  |  SCENE=  }}
-|RELATEDENTRY=[[1isn|1ISN]], [[1j19|1J19]], [[1e5w|1E5W]], [[1sgh|1SGH]], [[1ef1|1EF1]], [[1gc6|1GC6]], [[2i1j|2I1J]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1k OCA], [http://www.ebi.ac.uk/pdbsum/2i1k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i1k RCSB]</span>
-}}
 '''Moesin from Spodoptera frugiperda reveals the coiled-coil domain at 3.0 angstrom resolution'''
@@ Line 19: / Line 16: @@
 ==About this Structure==
-I1K is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1K OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1K OCA].
 ==Reference==
 Self-masking in an intact ERM-merlin protein: an active role for the central alpha-helical domain., Li Q, Nance MR, Kulikauskas R, Nyberg K, Fehon R, Karplus PA, Bretscher A, Tesmer JJ, J Mol Biol. 2007 Feb 2;365(5):1446-59. Epub 2006 Oct 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17134719 17134719]
-[[Category: Protein complex]]
-[[Category: Spodoptera frugiperda]]
 [[Category: Nance, M R.]]
 [[Category: Tesmer, J J.G.]]
-[[Category: actin binding]]
+[[Category: Actin binding]]
-[[Category: c-ermad]]
+[[Category: C-ermad]]
-[[Category: coiled-coil]]
+[[Category: Coiled-coil]]
-[[Category: erm]]
+[[Category: Erm]]
-[[Category: ezrin]]
+[[Category: Ezrin]]
-[[Category: ferm]]
+[[Category: Ferm]]
-[[Category: masking]]
+[[Category: Masking]]
-[[Category: merlin]]
+[[Category: Merlin]]
-[[Category: moesin]]
+[[Category: Moesin]]
-[[Category: radixin]]
+[[Category: Radixin]]
-[[Category: regulation]]
+[[Category: Regulation]]
-[[Category: self-inhibition]]
+[[Category: Self-inhibition]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:57:56 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:38:24 2008''