2hv8: Difference between revisions
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{{STRUCTURE_2hv8| PDB=2hv8 | SCENE= }} | |||
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'''Crystal structure of GTP-bound Rab11 in complex with FIP3''' | '''Crystal structure of GTP-bound Rab11 in complex with FIP3''' | ||
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[[Category: Mishra, A.]] | [[Category: Mishra, A.]] | ||
[[Category: Prekeris, R.]] | [[Category: Prekeris, R.]] | ||
[[Category: | [[Category: Cytokinesis]] | ||
[[Category: | [[Category: Fip3]] | ||
[[Category: | [[Category: Protein transport]] | ||
[[Category: | [[Category: Rab11a]] | ||
[[Category: | [[Category: Recycling endosome]] | ||
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Revision as of 06:45, 4 May 2008
Crystal structure of GTP-bound Rab11 in complex with FIP3
OverviewOverview
The Rab11 GTPase regulates recycling of internalized plasma membrane receptors and is essential for completion of cytokinesis. A family of Rab11 interacting proteins (FIPs) that conserve a C-terminal Rab-binding domain (RBD) selectively recognize the active form of Rab11. Normal completion of cytokinesis requires a complex between Rab11 and FIP3. Here, we report the crystal structure and mutational analysis of a heterotetrameric complex between constitutively active Rab11 and a FIP3 construct that includes the RBD. Two Rab11 molecules bind to dyad symmetric sites at the C terminus of FIP3, which forms a non-canonical coiled-coiled dimer with a flared C terminus and hook region. The RBD overlaps with the coiled coil and extends through the C-terminal hook. Although FIP3 engages the switch and interswitch regions of Rab11, the mode of interaction differs significantly from that of other Rab-effector complexes. In particular, the switch II region undergoes a large structural rearrangement from an ordered but non-complementary active conformation to a remodeled conformation that facilitates the interaction with FIP3. Finally, we provide evidence that FIP3 can form homo-oligomers in cells, and that a critical determinant of Rab11 binding in vitro is necessary for FIP3 recruitment to recycling endosomes during cytokinesis.
About this StructureAbout this Structure
2HV8 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for Rab11-mediated recruitment of FIP3 to recycling endosomes., Eathiraj S, Mishra A, Prekeris R, Lambright DG, J Mol Biol. 2006 Nov 24;364(2):121-35. Epub 2006 Aug 26. PMID:17007872 Page seeded by OCA on Sun May 4 06:45:08 2008