5oae: Difference between revisions
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==Crystal Structure of tyrosinase from Bacillus megaterium with SVF inhibitor in the active site== | ==Crystal Structure of tyrosinase from Bacillus megaterium with SVF inhibitor in the active site== | ||
<StructureSection load='5oae' size='340' side='right' caption='[[5oae]], [[Resolution|resolution]] 2.70Å' scene=''> | <StructureSection load='5oae' size='340' side='right'caption='[[5oae]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5oae]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OAE OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[5oae]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OAE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OAE FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=SVF:1-[4-[(4-fluorophenyl)methyl]piperidin-1-yl]ethanone'>SVF</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=SVF:1-[4-[(4-fluorophenyl)methyl]piperidin-1-yl]ethanone'>SVF</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5oae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oae OCA], [http://pdbe.org/5oae PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oae RCSB], [http://www.ebi.ac.uk/pdbsum/5oae PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oae ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5oae" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5oae" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Tyrosinase 3D structures|Tyrosinase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Atcc 14581]] | [[Category: Atcc 14581]] | ||
[[Category: Large Structures]] | |||
[[Category: Benhar, Y Pazy]] | [[Category: Benhar, Y Pazy]] | ||
[[Category: Deri, B]] | [[Category: Deri, B]] | ||
Revision as of 15:19, 26 August 2020
Crystal Structure of tyrosinase from Bacillus megaterium with SVF inhibitor in the active siteCrystal Structure of tyrosinase from Bacillus megaterium with SVF inhibitor in the active site
Structural highlights
Publication Abstract from PubMedThe inhibition of tyrosinase (Ty, EC 1.14.18.1) represents an efficient strategy of decreasing melanogenesis and skin hyperpigmentation. A combination of crystallographic and docking studies on two different tyrosinases, that from Bacillus megaterium (TyBm) and that from a mushroom (TyM), has contributed to increasing our knowledge about their structural information and translating that information to the most druggable human Ty (TyH) isozyme. In particular, we designed and synthesized a series of 1-(4-fluorobenzyl)piperazine and 1-(4-fluorobenzyl)piperidine derivatives showing inhibitory activities on TyM at micromolar ranges and more potency than that of the reference compound, kojic acid. The crystal structures of TyBm with inhibitor 3 (IC50 value of 25.11 muM) and 16 (IC50 value of 5.25 muM) were solved, confirming the binding poses hypothesized by in silico studies and revealing the main molecular determinants for the binding recognition of the inhibitors. Targeting Tyrosinase: Development and Structural Insights of Novel Inhibitors Bearing Arylpiperidine and Arylpiperazine Fragments.,Ferro S, Deri B, Germano MP, Gitto R, Ielo L, Buemi MR, Certo G, Vittorio S, Rapisarda A, Pazy Y, Fishman A, De Luca L J Med Chem. 2018 Apr 20. doi: 10.1021/acs.jmedchem.7b01745. PMID:29634898[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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