2hgh: Difference between revisions

Revision as of 06:15, 4 May 2008

Transcription Factor IIIA zinc fingers 4-6 bound to 5S rRNA 55mer (NMR structure)

OverviewOverview

Transcription factor IIIA (TFIIIA) is a Cys2His2 zinc finger protein that regulates expression of the 5 S ribosomal RNA gene by binding specifically to the internal control element. TFIIIA also functions in transport and storage of 5 S RNA by binding directly to the RNA transcript. To obtain insights into the mechanism by which TFIIIA recognizes 5 S RNA, we determined the solution structure of the middle three zinc fingers bound to the central core of 5 S RNA. Finger 4 utilizes "lock and key" recognition to bind in the widened major groove of the pre-structured RNA loop E motif. This interaction is mediated by direct hydrogen bonding interactions with bases. In contrast, recognition of loop A, a flexible junction of three helices, occurs by an induced fit mechanism that involves reorganization of the conserved CAUA motif and structuring of the finger 5-finger 6 interface to form a complementary RNA binding surface.

About this StructureAbout this Structure

2HGH is a Single protein structure of sequence from Xenopus laevis. The following page contains interesting information on the relation of 2HGH with [Zinc Fingers]. Full crystallographic information is available from OCA.

ReferenceReference

Induced fit and "lock and key" recognition of 5S RNA by zinc fingers of transcription factor IIIA., Lee BM, Xu J, Clarkson BK, Martinez-Yamout MA, Dyson HJ, Case DA, Gottesfeld JM, Wright PE, J Mol Biol. 2006 Mar 17;357(1):275-91. Epub 2005 Dec 20. PMID:16405997 Page seeded by OCA on Sun May 4 06:15:51 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2hgh.gif|left|200px]]
- {{Structure
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-|RELATEDENTRY=[[1tf3a|1TF3A]], [[1znf|1ZNF]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hgh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hgh OCA], [http://www.ebi.ac.uk/pdbsum/2hgh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hgh RCSB]</span>
-}}
 '''Transcription Factor IIIA zinc fingers 4-6 bound to 5S rRNA 55mer (NMR structure)'''
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 [[Category: Zinc Fingers]]
 [[Category: Lee, B M.]]
-[[Category: zinc finger]]
+[[Category: Zinc finger]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:15:51 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:30:01 2008''