2h0x: Difference between revisions

Revision as of 05:44, 4 May 2008

Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme

OverviewOverview

The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis.

About this StructureAbout this Structure

2H0X is a Single protein structure. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:16990543 Page seeded by OCA on Sun May 4 05:44:18 2008

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OCA

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 [[Image:2h0x.gif|left|200px]]
- {{Structure
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-|DOMAIN=
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-|RELATEDENTRY=[[2gcs|2GCS]], [[2gcv|2GCV]], [[2h0s|2H0S]], [[2h0w|2H0W]], [[2h0z|2H0Z]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h0x OCA], [http://www.ebi.ac.uk/pdbsum/2h0x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h0x RCSB]</span>
-}}
 '''Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme'''
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 ==About this Structure==
-H0X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H0X OCA].
+H0X is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H0X OCA].
 ==Reference==
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 [[Category: Amare, A R.Ferre-D.]]
 [[Category: Klein, D J.]]
-[[Category: helix]]
+[[Category: Helix]]
-[[Category: pseudoknot]]
+[[Category: Pseudoknot]]
-[[Category: ribozyme]]
+[[Category: Ribozyme]]
-[[Category: rna]]
+[[Category: Rna]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:44:18 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:23:53 2008''