1ww4: Difference between revisions
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==Agrocybe cylindracea galectin complexed with NeuAca2-3lactose== | ==Agrocybe cylindracea galectin complexed with NeuAca2-3lactose== | ||
<StructureSection load='1ww4' size='340' side='right' caption='[[1ww4]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='1ww4' size='340' side='right'caption='[[1ww4]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1ww4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrocybe_cylindracea Agrocybe cylindracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WW4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WW4 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1ww4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrocybe_cylindracea Agrocybe cylindracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WW4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WW4 FirstGlance]. <br> | ||
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==See Also== | ==See Also== | ||
*[[Galectin|Galectin]] | *[[Galectin 3D structures|Galectin 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Agrocybe cylindracea]] | [[Category: Agrocybe cylindracea]] | ||
[[Category: Large Structures]] | |||
[[Category: Ban, M]] | [[Category: Ban, M]] | ||
[[Category: Demirkan, E]] | [[Category: Demirkan, E]] | ||
Revision as of 20:43, 22 January 2020
Agrocybe cylindracea galectin complexed with NeuAca2-3lactoseAgrocybe cylindracea galectin complexed with NeuAca2-3lactose
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedGalectin from an edible fungus Agrocybe cylindracea (ACG) has a strong preference for N-acetylneuraminyl lactose (NeuAcalpha2-3lactose). The sugar recognition mechanism of ACG was explored by the X-ray crystallographic analyses of ligand-free ACG, and its complex with lactose, 3'-sulfonyl lactose and NeuAcalpha2-3lactose. The refined structure shows that ACG is a "proto"-type galectin composed of a beta-sandwich of two antiparallel sheets, each with six strands, in contrast to the five and six strands in animal galectins. ACG dimer in solution was classified as being among the "layer"-type. The carbohydrate recognition domain (CRD) of this galectin is common to those of animal galectins, except for substitution of one residue, Ala64, which corresponds to Asn46 in human galectin 1. A five-residue insertion in ACG at positions 42-46 involving Ser44 and Asn46 modified the architecture of the sugar binding site that contributes sialic acid specificity. Furthermore, it was found that the binding of a sulfate ion near the CRD in the ligand-free form led to a change in the conformation of the loop region caused by main-chain cis/trans transition between Ser44 and Pro45. Structural basis of a fungal galectin from Agrocybe cylindracea for recognizing sialoconjugate.,Ban M, Yoon HJ, Demirkan E, Utsumi S, Mikami B, Yagi F J Mol Biol. 2005 Aug 26;351(4):695-706. PMID:16051274[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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