2gjw: Difference between revisions

Revision as of 05:11, 4 May 2008

RNA Recognition and Cleavage by an Splicing Endonuclease

OverviewOverview

The RNA splicing endonuclease cleaves two phosphodiester bonds within folded precursor RNAs during intron removal, producing the functional RNAs required for protein synthesis. Here we describe at a resolution of 2.85 angstroms the structure of a splicing endonuclease from Archaeglobus fulgidus bound with a bulge-helix-bulge RNA containing a noncleaved and a cleaved splice site. The endonuclease dimer cooperatively recognized a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line RNA cleavage reaction. Cooperativity arises because an arginine pair from one catalytic domain sandwiches a nucleobase within the bulge cleaved by the other catalytic domain.

About this StructureAbout this Structure

2GJW is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

ReferenceReference

RNA recognition and cleavage by a splicing endonuclease., Xue S, Calvin K, Li H, Science. 2006 May 12;312(5775):906-10. PMID:16690865 Page seeded by OCA on Sun May 4 05:11:22 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2gjw.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2gjw |SIZE=350|CAPTION= <scene name='initialview01'>2gjw</scene>, resolution 2.85&Aring;
+The line below this paragraph, containing "STRUCTURE_2gjw", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=A23:ADENOSINE-5&#39;-PHOSPHATE-2&#39;,3&#39;-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=DU:2&#39;-DEOXYURIDINE-5&#39;-MONOPHOSPHATE'>DU</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= endA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus])
+-->
-|DOMAIN=
+{{STRUCTURE_2gjw|  PDB=2gjw  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjw OCA], [http://www.ebi.ac.uk/pdbsum/2gjw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gjw RCSB]</span>
-}}
 '''RNA Recognition and Cleavage by an Splicing Endonuclease'''
@@ Line 25: / Line 22: @@
 [[Category: Archaeoglobus fulgidus]]
 [[Category: Single protein]]
-[[Category: tRNA-intron endonuclease]]
+[[Category: TRNA-intron endonuclease]]
 [[Category: Calvin, K.]]
 [[Category: Li, H.]]
 [[Category: Xue, S.]]
-[[Category: bulge-helix-bulge rna-protein complex]]
+[[Category: Bulge-helix-bulge rna-protein complex]]
-[[Category: splicing endonuclease af]]
+[[Category: Splicing endonuclease af]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:11:22 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:17:38 2008''