2gig: Difference between revisions
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'''Alteration of sequence specificity of the type II restriction endonuclease HINCII through an indirect readout mechanism''' | '''Alteration of sequence specificity of the type II restriction endonuclease HINCII through an indirect readout mechanism''' | ||
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[[Category: Horton, N C.]] | [[Category: Horton, N C.]] | ||
[[Category: Joshi, H K.]] | [[Category: Joshi, H K.]] | ||
[[Category: | [[Category: Dna intercalation]] | ||
[[Category: | [[Category: Endonuclease]] | ||
[[Category: | [[Category: Indirect readout]] | ||
[[Category: | [[Category: Protein dna complex]] | ||
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Revision as of 05:08, 4 May 2008
Alteration of sequence specificity of the type II restriction endonuclease HINCII through an indirect readout mechanism
OverviewOverview
The functional and structural consequences of a mutation of the DNA intercalating residue of HincII, Q138F, are presented. Modeling has suggested that the DNA intercalation by Gln-138 results in DNA distortions potentially used by HincII in indirect readout of its cognate DNA, GTYRAC (Y = C or T, R = A or G) (Horton, N. C., Dorner, L. F., and Perona, J. J. (2002) Nat. Struct. Biol. 9, 42-47). Kinetic data presented here indicate that the mutation of glutamine 138 to phenylalanine (Q138F) results in a change in sequence specificity at the center two base pairs of the cognate recognition site. We show that the preference of HincII for cutting, but not binding, the three cognate sites differing in the center two base pairs has been altered by the mutation Q138F. Five new crystal structures are presented including Q138F HincII bound to GTTAAC and GTCGAC both with and without Ca2+ as well as the structure of wild type HincII bound to GTTAAC. The Q138F HincII/DNA structures show conformational changes in the protein, bound DNA, and at the protein-DNA interface, consistent with the formation of adaptive complexes. Analysis of these structures and the effect of Ca2+ binding on the protein-DNA interface illuminates the origin of the altered specificity by the mutation Q138F in the HincII enzyme.
About this StructureAbout this Structure
2GIG is a Single protein structure of sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.
ReferenceReference
Alteration of sequence specificity of the type II restriction endonuclease HincII through an indirect readout mechanism., Joshi HK, Etzkorn C, Chatwell L, Bitinaite J, Horton NC, J Biol Chem. 2006 Aug 18;281(33):23852-69. Epub 2006 May 4. PMID:16675462 Page seeded by OCA on Sun May 4 05:08:36 2008