2fx4: Difference between revisions

Revision as of 04:24, 4 May 2008

Bovine trypsin bound by 4-piperidinebutyrate to make acylenzyme complex

OverviewOverview

Improved peptide-based inhibitors of human beta tryptase were discovered using information gleaned from tripeptide library screening and structure-guided design methods, including fragment screening. Our efforts sought to improve this class of inhibitors by replacing the traditional Lys or Arg P1 element. The optimized compounds display low nanomolar potency against the mast cell target and several hundred-fold selectivity with respect to serine protease off targets. Thus, replacement of Lys/Arg at P1 in a peptide-like scaffold does not need to be accompanied by a loss in target affinity.

About this StructureAbout this Structure

2FX4 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

Structure-guided design of peptide-based tryptase inhibitors., McGrath ME, Sprengeler PA, Hirschbein B, Somoza JR, Lehoux I, Janc JW, Gjerstad E, Graupe M, Estiarte A, Venkataramani C, Liu Y, Yee R, Ho JD, Green MJ, Lee CS, Liu L, Tai V, Spencer J, Sperandio D, Katz BA, Biochemistry. 2006 May 16;45(19):5964-73. PMID:16681368 Page seeded by OCA on Sun May 4 04:24:29 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2fx4.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2fx4 |SIZE=350|CAPTION= <scene name='initialview01'>2fx4</scene>, resolution 1.65&Aring;
+The line below this paragraph, containing "STRUCTURE_2fx4", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=C1R:4-PIPERIDINEBUTYRATE'>C1R</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2fx4|  PDB=2fx4  |  SCENE=  }}
-|RELATEDENTRY=[[2fww|2FWW]], [[2fx6|2FX6]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fx4 OCA], [http://www.ebi.ac.uk/pdbsum/2fx4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fx4 RCSB]</span>
-}}
 '''Bovine trypsin bound by 4-piperidinebutyrate to make acylenzyme complex'''
@@ Line 27: / Line 24: @@
 [[Category: Trypsin]]
 [[Category: Katz, B A.]]
-[[Category: acylenzyme complex]]
+[[Category: Acylenzyme complex]]
-[[Category: serine protease]]
+[[Category: Serine protease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:24:29 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:08:53 2008''