2fs3: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2fs3.gif|left|200px]] | [[Image:2fs3.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_2fs3", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_2fs3| PDB=2fs3 | SCENE= }} | |||
| | |||
}} | |||
'''Bacteriophage HK97 K169Y Head I''' | '''Bacteriophage HK97 K169Y Head I''' | ||
| Line 35: | Line 32: | ||
[[Category: Liljas, L.]] | [[Category: Liljas, L.]] | ||
[[Category: Speir, J A.]] | [[Category: Speir, J A.]] | ||
[[Category: | [[Category: Bacteriophage]] | ||
[[Category: | [[Category: Capsid]] | ||
[[Category: | [[Category: Expansion intermediate]] | ||
[[Category: | [[Category: Hk97]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:14:41 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 04:14, 4 May 2008
Bacteriophage HK97 K169Y Head I
OverviewOverview
Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II) to the mature state (Head II) involves a 60 A radial expansion. The mature particle is formed by 420 copies of the major capsid protein organized on a T = 7 laevo lattice with each subunit covalently crosslinked to two neighbors. Well-characterized pH 4 expansion intermediates make HK97 valuable for investigating quaternary structural dynamics. Here, we use X-ray crystallography and cryo-EM to demonstrate that in the final transition in maturation (requiring neutral pH), pentons in Expansion Intermediate IV (EI-IV) reversibly sample 14 A translations and 6 degrees rotations relative to a fixed hexon lattice. The limit of this trajectory corresponds to the Head II conformation that is secured at this extent only by the formation of the final class of covalent crosslinks. Mutants that cannot crosslink or EI-IV particles that have been rendered incapable of forming the final crosslink remain in the EI-IV state.
About this StructureAbout this Structure
2FS3 is a Single protein structure of sequence from Enterobacteria phage hk97. Full crystallographic information is available from OCA.
ReferenceReference
Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM., Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE, Structure. 2006 Nov;14(11):1655-65. PMID:17098191 Page seeded by OCA on Sun May 4 04:14:41 2008