2frf: Difference between revisions

Revision as of 04:13, 4 May 2008

Horse Heart Myoglobin, Nitrite Adduct, Crystal Soak

OverviewOverview

Nitrite is an important species in the global nitrogen cycle, and the nitrite reductase enzymes convert nitrite to nitric oxide (NO). Recently, it has been shown that hemoglobin and myoglobin catalyze the reduction of nitrite to NO under hypoxic conditions. We have determined the 1.20 A resolution crystal structure of the nitrite adduct of ferric horse heart myoglobin (hh Mb). The ligand is bound to iron in the nitrito form, and the complex is formulated as MbIII(ONO-). The Fe-ONO bond length is 1.94 A, and the O-N-O angle is 113 degrees . In addition, the nitrite ligand is stabilized by hydrogen bonding with the distal His64 residue. We have also determined the 1.30 A resolution crystal structures of hh MbIINO. When hh MbIINO is prepared from the reaction of metMbIII with nitrite/dithionite, the FeNO angle is 144 degrees with a Fe-NO bond length of 1.87 A. However, when prepared from the reaction of NO with reduced MbII, the FeNO angle is 120 degrees with a Fe-NO bond length of 2.13 A. This difference in FeNO conformations as a function of preparative method is reproducible, and suggests a role of the distal pocket in hh MbIINO in stabilizing local FeNO conformational minima.

About this StructureAbout this Structure

2FRF is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of the nitrite and nitric oxide complexes of horse heart myoglobin., Copeland DM, Soares AS, West AH, Richter-Addo GB, J Inorg Biochem. 2006 Aug;100(8):1413-25. Epub 2006 May 5. PMID:16777231 Page seeded by OCA on Sun May 4 04:13:41 2008

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OCA

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 [[Image:2frf.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2frf |SIZE=350|CAPTION= <scene name='initialview01'>2frf</scene>, resolution 1.20&Aring;
+The line below this paragraph, containing "STRUCTURE_2frf", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO2:NITRITE+ION'>NO2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2frf|  PDB=2frf  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2frf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2frf OCA], [http://www.ebi.ac.uk/pdbsum/2frf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2frf RCSB]</span>
-}}
 '''Horse Heart Myoglobin, Nitrite Adduct, Crystal Soak'''
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 [[Category: Soares, A S.]]
 [[Category: West, A H.]]
-[[Category: heme]]
+[[Category: Heme]]
-[[Category: iron]]
+[[Category: Iron]]
-[[Category: myoglobin]]
+[[Category: Myoglobin]]
-[[Category: nitric oxide]]
+[[Category: Nitric oxide]]
-[[Category: nitrite]]
+[[Category: Nitrite]]
-[[Category: no]]
+[[Category: No]]
-[[Category: no2]]
+[[Category: No2]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:13:41 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:06:43 2008''