2fop: Difference between revisions

Revision as of 04:08, 4 May 2008

The Crystal Strucure of the N-terminal domain of HAUSP/USP7 complexed with mdm2 peptide 147-150

OverviewOverview

The ubiquitin-specific protease, USP7, has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. We show that the N-terminal domain of USP7 binds two closely spaced 4-residue sites in both p53 and MDM2, falling between p53 residues 359-367 and MDM2 residues 147-159. Cocrystal structures with USP7 were determined for both p53 peptides and for one MDM2 peptide. These peptides bind the same surface of USP7 as Epstein-Barr nuclear antigen-1, explaining the competitive nature of the interactions. The structures and mutagenesis data indicate a preference for a P/AXXS motif in peptides that bind USP7. Contacts made by serine are identical and crucial for all peptides, and Trp165 in the peptide-binding pocket of USP7 is also crucial. These results help to elucidate the mechanism of substrate recognition by USP7 and the regulation of the p53 pathway.

About this StructureAbout this Structure

2FOP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Molecular recognition of p53 and MDM2 by USP7/HAUSP., Sheng Y, Saridakis V, Sarkari F, Duan S, Wu T, Arrowsmith CH, Frappier L, Nat Struct Mol Biol. 2006 Mar;13(3):285-91. Epub 2006 Feb 12. PMID:16474402 Page seeded by OCA on Sun May 4 04:08:43 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2fop.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2fop |SIZE=350|CAPTION= <scene name='initialview01'>2fop</scene>, resolution 2.10&Aring;
+The line below this paragraph, containing "STRUCTURE_2fop", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin_thiolesterase Ubiquitin thiolesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.15 3.1.2.15] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= HAUSP, USP7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_2fop|  PDB=2fop  |  SCENE=  }}
-|RELATEDENTRY=[[2foo|2FOO]], [[2foj|2FOJ]], [[1yy6|1YY6]], [[1yze|1YZE]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fop OCA], [http://www.ebi.ac.uk/pdbsum/2fop PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fop RCSB]</span>
-}}
 '''The Crystal Strucure of the N-terminal domain of HAUSP/USP7 complexed with mdm2 peptide 147-150'''
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 [[Category: Sheng, Y.]]
 [[Category: Wu, T.]]
-[[Category: math domain]]
+[[Category: Math domain]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:08:43 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:05:43 2008''