Nucleoplasmin: Difference between revisions
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= Activation by Phosphorylation = | |||
NP decondensate the chromatin by the poly-glu tail, Phosphorylation on the core domain is also required for efficient decondensation. Partial phosphorylation can give the protein the ability to bind core histones, but only hyperphosphorylation of specific regions of both protein domains can make the decondensation process to begin. The hyperphosphorylation is correlated with an enhanced H2A/H2B dimer exchange activity during the decondensation of sperm chromatin. | NP decondensate the chromatin by the poly-glu tail, Phosphorylation on the core domain is also required for efficient decondensation. Partial phosphorylation can give the protein the ability to bind core histones, but only hyperphosphorylation of specific regions of both protein domains can make the decondensation process to begin. The hyperphosphorylation is correlated with an enhanced H2A/H2B dimer exchange activity during the decondensation of sperm chromatin. | ||
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= | = conservation = | ||
The loop in the core domain of Xenopus NP is not highly conserved, [[Image:1k5j.png | thumb | Xenopus]] | The loop in the core domain of Xenopus NP is not highly conserved, [[Image:1k5j.png | thumb | Xenopus]] | ||
while the core domain in human is more conserved compared to Xenopus. [[Image:3T30.png | thumb | Human]] | while the core domain in human is more conserved compared to Xenopus. [[Image:3T30.png | thumb | Human]] | ||
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=3D structures of nucleoplasmin= | |||
[[1k5j]] – XlNpl core – ''Xenopus laevis'' | [[1k5j]] – XlNpl core – ''Xenopus laevis'' | ||
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[[3t30]] - Human nucleoplasmin | [[3t30]] - Human nucleoplasmin | ||
= References = | |||
<references/> | <references/> | ||
2. Taneva SG1, Muñoz IG, Franco G, Falces J, Arregi I, Muga A, Montoya G, Urbaneja MA, Bañuelos S. Activation of nucleoplasmin, an oligomeric histone chaperone, challenges its stability.Biochemistry. 2008 Dec 30;47(52):13897-906. PMID:19055325 DOI:10.1021/bi800975r | 2. Taneva SG1, Muñoz IG, Franco G, Falces J, Arregi I, Muga A, Montoya G, Urbaneja MA, Bañuelos S. Activation of nucleoplasmin, an oligomeric histone chaperone, challenges its stability.Biochemistry. 2008 Dec 30;47(52):13897-906. PMID:19055325 DOI:10.1021/bi800975r | ||