1se9: Difference between revisions
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==Structure of At3g01050, a ubiquitin-fold protein from Arabidopsis thaliana== | ==Structure of At3g01050, a ubiquitin-fold protein from Arabidopsis thaliana== | ||
<StructureSection load='1se9' size='340' side='right' caption='[[1se9]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | <StructureSection load='1se9' size='340' side='right'caption='[[1se9]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1se9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE9 OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[1se9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1SE9 FirstGlance]. <br> | ||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At3g01050 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At3g01050 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1se9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se9 OCA], [http://pdbe.org/1se9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1se9 RCSB], [http://www.ebi.ac.uk/pdbsum/1se9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1se9 ProSAT], [http://www.topsan.org/Proteins/CESG/1se9 TOPSAN]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Arath]] | [[Category: Arath]] | ||
[[Category: Large Structures]] | |||
[[Category: Structural genomic]] | [[Category: Structural genomic]] | ||
[[Category: Lytle, B L]] | [[Category: Lytle, B L]] | ||
Revision as of 14:21, 24 December 2020
Structure of At3g01050, a ubiquitin-fold protein from Arabidopsis thalianaStructure of At3g01050, a ubiquitin-fold protein from Arabidopsis thaliana
Structural highlights
Function[MUB1_ARATH] May serve as docking site to facilitate the association of other proteins to the plasma membrane. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedStructural proteomics requires robust, scalable methods. Here we describe a wheat germ cell-free platform for protein production that supports efficient NMR structural studies of eukaryotic proteins and offers advantages over cell-based methods. To illustrate this platform, we describe its application to a specific target (At3g01050.1) from Arabidopsis thaliana. After cloning the target gene into a specialized plasmid, we carry out a small-scale (50 mul) in vitro sequential transcription and translation trial to ascertain the level of protein production and solubility. Next, we prepare mRNA for use in a 4-ml semicontinuous cell-free translation reaction to incorporate (15)N-labeled amino acids into a protein sample that we purify and test for suitability for NMR structural analysis. We then repeat the cell-free approach with (13)C,(15)N-labeled amino acids to prepare a doubly labeled sample. The three-dimensional (3D) structure of At3g01050.1 shows that this protein is an unusual member of the beta-grasp protein family. Cell-free protein production and labeling protocol for NMR-based structural proteomics.,Vinarov DA, Lytle BL, Peterson FC, Tyler EM, Volkman BF, Markley JL Nat Methods. 2004 Nov;1(2):149-53. Epub 2004 Oct 21. PMID:15782178[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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