2ffa: Difference between revisions

Revision as of 03:49, 4 May 2008

Crystal structure of ABC-ATPase H662A of the ABC-transporter HlyB in complex with ADP

OverviewOverview

The ATP-binding cassette (ABC)-transporter haemolysin (Hly)B, a central element of a Type I secretion machinery, acts in concert with two additional proteins in Escherichia coli to translocate the toxin HlyA directly from the cytoplasm to the exterior. The basic set of crystal structures necessary to describe the catalytic cycle of the isolated HlyB-NBD (nucleotide-binding domain) has now been completed. This allowed a detailed analysis with respect to hinge regions, functionally important key residues and potential energy storage devices that revealed many novel features. These include a structural asymmetry within the ATP dimer that was significantly enhanced in the presence of Mg2+, indicating a possible functional asymmetry in the form of one open and one closed phosphate exit tunnel. Guided by the structural analysis, we identified two amino acids, closing one tunnel by an apparent salt bridge. Mutation of these residues abolished ATP-dependent cooperativity of the NBDs. The implications of these new findings for the coupling of ATP binding and hydrolysis to functional activity are discussed.

About this StructureAbout this Structure

2FFA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

A structural analysis of asymmetry required for catalytic activity of an ABC-ATPase domain dimer., Zaitseva J, Oswald C, Jumpertz T, Jenewein S, Wiedenmann A, Holland IB, Schmitt L, EMBO J. 2006 Jul 26;25(14):3432-43. Epub 2006 Jul 6. PMID:16858415 Page seeded by OCA on Sun May 4 03:49:33 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2ffa.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2ffa |SIZE=350|CAPTION= <scene name='initialview01'>2ffa</scene>, resolution 1.70&Aring;
+The line below this paragraph, containing "STRUCTURE_2ffa", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= hlyB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_2ffa|  PDB=2ffa  |  SCENE=  }}
-|RELATEDENTRY=[[1mt0|1MT0]], [[1xef|1XEF]], [[2ff7|2FF7]], [[2ffb|2FFB]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ffa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ffa OCA], [http://www.ebi.ac.uk/pdbsum/2ffa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ffa RCSB]</span>
-}}
 '''Crystal structure of ABC-ATPase H662A of the ABC-transporter HlyB in complex with ADP'''
@@ Line 31: / Line 28: @@
 [[Category: Schmitt, L.]]
 [[Category: Zaitseva, J.]]
-[[Category: abc-transporter]]
+[[Category: Abc-transporter]]
-[[Category: atpase]]
+[[Category: Atpase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:49:33 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:02:02 2008''