5cr4: Difference between revisions
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==Crystal structure of the Sleeping Beauty transposase catalytic domain== | ==Crystal structure of the Sleeping Beauty transposase catalytic domain== | ||
<StructureSection load='5cr4' size='340' side='right' caption='[[5cr4]], [[Resolution|resolution]] 1.40Å' scene=''> | <StructureSection load='5cr4' size='340' side='right'caption='[[5cr4]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5cr4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CR4 FirstGlance]. <br> | <table><tr><td colspan='2'>[[5cr4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CR4 FirstGlance]. <br> | ||
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==See Also== | ==See Also== | ||
*[[Transposase|Transposase]] | *[[Transposase 3D structures|Transposase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct sequences]] | [[Category: Synthetic construct sequences]] | ||
[[Category: Barabas, O]] | [[Category: Barabas, O]] | ||
Revision as of 14:51, 27 March 2020
Crystal structure of the Sleeping Beauty transposase catalytic domainCrystal structure of the Sleeping Beauty transposase catalytic domain
Structural highlights
Publication Abstract from PubMedSleeping Beauty (SB) is a prominent Tc1/mariner superfamily DNA transposon that provides a popular genome engineering tool in a broad range of organisms. It is mobilized by a transposase enzyme that catalyses DNA cleavage and integration at short specific sequences at the transposon ends. To facilitate SB's applications, here we determine the crystal structure of the transposase catalytic domain and use it to model the SB transposase/transposon end/target DNA complex. Together with biochemical and cell-based transposition assays, our structure reveals mechanistic insights into SB transposition and rationalizes previous hyperactive transposase mutations. Moreover, our data enables us to design two additional hyperactive transposase variants. Our work provides a useful resource and proof-of-concept for structure-based engineering of tailored SB transposases. Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering.,Voigt F, Wiedemann L, Zuliani C, Querques I, Sebe A, Mates L, Izsvak Z, Ivics Z, Barabas O Nat Commun. 2016 Mar 30;7:11126. doi: 10.1038/ncomms11126. PMID:27025571[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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