2fek: Difference between revisions

Revision as of 03:48, 4 May 2008

Structure of a protein tyrosine phosphatase

OverviewOverview

Low molecular weight protein-tyrosine phosphatases (LMW-PTPs) are small enzymes that ubiquitously exist in various organisms and play important roles in many biological processes. In Escherichia coli, the LMW-PTP Wzb dephosphorylates the autokinase Wzc, and the Wzc/Wzb pair regulates colanic acid production. However, the substrate recognition mechanism of Wzb is still poorly understood thus far. To elucidate the molecular basis of the catalytic mechanism, we have determined the solution structure of Wzb at high resolution by NMR spectroscopy. The Wzb structure highly resembles that of the typical LMW-PTP fold, suggesting that Wzb may adopt a similar catalytic mechanism with other LMW-PTPs. Nevertheless, in comparison with eukaryotic LMW-PTPs, the absence of an aromatic amino acid at the bottom of the active site significantly alters the molecular surface and implicates Wzb may adopt a novel substrate recognition mechanism. Furthermore, a structure-based multiple sequence alignment suggests that a class of the prokaryotic LMW-PTPs may share a similar substrate recognition mechanism with Wzb. The current studies provide the structural basis for rational drug design against the pathogenic bacteria.

About this StructureAbout this Structure

2FEK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

The solution structure of Escherichia coli Wzb reveals a novel substrate recognition mechanism of prokaryotic low molecular weight protein-tyrosine phosphatases., Lescop E, Hu Y, Xu H, Hu W, Chen J, Xia B, Jin C, J Biol Chem. 2006 Jul 14;281(28):19570-7. Epub 2006 May 1. PMID:16651264 Page seeded by OCA on Sun May 4 03:48:16 2008

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OCA

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 [[Image:2fek.gif|left|200px]]
- {{Structure
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span>
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-|GENE= wzb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fek OCA], [http://www.ebi.ac.uk/pdbsum/2fek PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fek RCSB]</span>
-}}
 '''Structure of a protein tyrosine phosphatase'''
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 [[Category: Jin, C.]]
 [[Category: Lescop, E.]]
-[[Category: escherichia coli]]
+[[Category: Escherichia coli]]
-[[Category: low molecular weight protein tyrosine phosphatase]]
+[[Category: Low molecular weight protein tyrosine phosphatase]]
-[[Category: phosphate binding]]
+[[Category: Phosphate binding]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:48:16 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:01:44 2008''