2f49: Difference between revisions

Revision as of 03:26, 4 May 2008

Crystal structure of Fus3 in complex with a Ste5 peptide

OverviewOverview

Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active-it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties.

About this StructureAbout this Structure

2F49 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway., Bhattacharyya RP, Remenyi A, Good MC, Bashor CJ, Falick AM, Lim WA, Science. 2006 Feb 10;311(5762):822-6. Epub 2006 Jan 19. PMID:16424299 Page seeded by OCA on Sun May 4 03:26:43 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2f49.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2f49 |SIZE=350|CAPTION= <scene name='initialview01'>2f49</scene>, resolution 1.9&Aring;
+The line below this paragraph, containing "STRUCTURE_2f49", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene>
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= FUS3, DAC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+-->
-|DOMAIN=
+{{STRUCTURE_2f49|  PDB=2f49  |  SCENE=  }}
-|RELATEDENTRY=[[2b9f|2B9F]], [[2b9h|2B9H]], [[2b9i|2B9I]], [[2b9j|2B9J]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f49 OCA], [http://www.ebi.ac.uk/pdbsum/2f49 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f49 RCSB]</span>
-}}
 '''Crystal structure of Fus3 in complex with a Ste5 peptide'''
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 [[Category: Single protein]]
 [[Category: Remenyi, A.]]
-[[Category: protein-petide complex]]
+[[Category: Protein-petide complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:26:43 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:57:42 2008''