2f2h: Difference between revisions

Revision as of 03:23, 4 May 2008

Structure of the YicI thiosugar Michaelis complex

OverviewOverview

For the first time, the thioglycoligase strategy has been successfully applied to alpha-glycosidases. The alpha-thioglycoligases derived from the family 31 glycosidases, alpha-xylosidase from E. coli (YicI) and alpha-glucosidase from Sulfolobus solfataricus, catalyze thioglycoligase reactions using alpha-glycosyl fluorides and deoxythioglycosides as donors and acceptors, respectively, in yields up to 86%. In addition, we describe the Michaelis complex of YicI using one of the thioglycosides as a nonhydrolyzable substrate analogue and discuss the structural insights this yields into the specificity and mechanism of family 31 alpha-glycosidases and the molecular basis of an associated genetic disease.

About this StructureAbout this Structure

2F2H is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex., Kim YW, Lovering AL, Chen H, Kantner T, McIntosh LP, Strynadka NC, Withers SG, J Am Chem Soc. 2006 Feb 22;128(7):2202-3. PMID:16478160 Page seeded by OCA on Sun May 4 03:23:13 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2f2h.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2f2h |SIZE=350|CAPTION= <scene name='initialview01'>2f2h</scene>, resolution 1.950&Aring;
+The line below this paragraph, containing "STRUCTURE_2f2h", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XTG:4-NITROPHENYL+6-THIO-6-S-ALPHA-D-XYLOPYRANOSYL-BETA-D-GLUCOPYRANOSIDE'>XTG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= yicI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_2f2h|  PDB=2f2h  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f2h OCA], [http://www.ebi.ac.uk/pdbsum/2f2h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f2h RCSB]</span>
-}}
 '''Structure of the YicI thiosugar Michaelis complex'''
@@ Line 29: / Line 26: @@
 [[Category: Strynadka, N C.J.]]
 [[Category: Withers, S G.]]
-[[Category: beta8alpha8 barrel]]
+[[Category: Beta8alpha8 barrel]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:23:13 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:57:00 2008''