6fc3: Difference between revisions

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'''Unreleased structure'''


The entry 6fc3 is ON HOLD until Paper Publication
==Crystal structure of the eIF4E-p20 complex from Saccharomyces cerevisiae==
 
<StructureSection load='6fc3' size='340' side='right' caption='[[6fc3]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
Authors:  
== Structural highlights ==
 
<table><tr><td colspan='2'>[[6fc3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FC3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FC3 FirstGlance]. <br>
Description:  
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
[[Category: Unreleased Structures]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fc3 OCA], [http://pdbe.org/6fc3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fc3 RCSB], [http://www.ebi.ac.uk/pdbsum/6fc3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fc3 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/IF4E_YEAST IF4E_YEAST]] Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. [[http://www.uniprot.org/uniprot/CAF20_YEAST CAF20_YEAST]] Acts as an inhibitor of cap-dependent translation. Competes with eIF4G1/TIF4631 and EAP1 for binding to eIF4E/TIF45 and interferes with the formation of the eIF4F complex, inhibiting translation and stabilizing mRNA. Binding affinity for eIF4E/TIF45 is 10-fold less than that of eIF4G1/TIF4631. Required for induction of pseudohyphal growth in response to nitrogen limitation, probably by regulating STE12 translation.<ref>PMID:10848587</ref> <ref>PMID:12054793</ref> <ref>PMID:12406227</ref> <ref>PMID:17041186</ref> <ref>PMID:17083129</ref> <ref>PMID:9118949</ref> <ref>PMID:9707439</ref>  
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gruener, S]]
[[Category: Valkov, E]]
[[Category: Translation]]
[[Category: Translation translational control gene expression 4e-binding protein]]

Revision as of 08:40, 27 June 2018

Crystal structure of the eIF4E-p20 complex from Saccharomyces cerevisiaeCrystal structure of the eIF4E-p20 complex from Saccharomyces cerevisiae

Structural highlights

6fc3 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IF4E_YEAST] Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. [CAF20_YEAST] Acts as an inhibitor of cap-dependent translation. Competes with eIF4G1/TIF4631 and EAP1 for binding to eIF4E/TIF45 and interferes with the formation of the eIF4F complex, inhibiting translation and stabilizing mRNA. Binding affinity for eIF4E/TIF45 is 10-fold less than that of eIF4G1/TIF4631. Required for induction of pseudohyphal growth in response to nitrogen limitation, probably by regulating STE12 translation.[1] [2] [3] [4] [5] [6] [7]

References

  1. ↑ Cosentino GP, Schmelzle T, Haghighat A, Helliwell SB, Hall MN, Sonenberg N. Eap1p, a novel eukaryotic translation initiation factor 4E-associated protein in Saccharomyces cerevisiae. Mol Cell Biol. 2000 Jul;20(13):4604-13. PMID:10848587
  2. ↑ Ramirez CV, Vilela C, Berthelot K, McCarthy JE. Modulation of eukaryotic mRNA stability via the cap-binding translation complex eIF4F. J Mol Biol. 2002 May 10;318(4):951-62. PMID:12054793 doi:http://dx.doi.org/10.1016/S0022-2836(02)00162-6
  3. ↑ von der Haar T, McCarthy JE. Intracellular translation initiation factor levels in Saccharomyces cerevisiae and their role in cap-complex function. Mol Microbiol. 2002 Oct;46(2):531-44. PMID:12406227
  4. ↑ Park YU, Hur H, Ka M, Kim J. Identification of translational regulation target genes during filamentous growth in Saccharomyces cerevisiae: regulatory role of Caf20 and Dhh1. Eukaryot Cell. 2006 Dec;5(12):2120-7. Epub 2006 Oct 13. PMID:17041186 doi:http://dx.doi.org/EC.00121-06
  5. ↑ Ibrahimo S, Holmes LE, Ashe MP. Regulation of translation initiation by the yeast eIF4E binding proteins is required for the pseudohyphal response. Yeast. 2006 Oct-Nov;23(14-15):1075-88. PMID:17083129 doi:http://dx.doi.org/10.1002/yea.1415
  6. ↑ Altmann M, Schmitz N, Berset C, Trachsel H. A novel inhibitor of cap-dependent translation initiation in yeast: p20 competes with eIF4G for binding to eIF4E. EMBO J. 1997 Mar 3;16(5):1114-21. doi: 10.1093/emboj/16.5.1114. PMID:9118949 doi:http://dx.doi.org/10.1093/emboj/16.5.1114
  7. ↑ Ptushkina M, von der Haar T, Vasilescu S, Frank R, Birkenhager R, McCarthy JE. Cooperative modulation by eIF4G of eIF4E-binding to the mRNA 5' cap in yeast involves a site partially shared by p20. EMBO J. 1998 Aug 17;17(16):4798-808. PMID:9707439 doi:http://dx.doi.org/10.1093/emboj/17.16.4798

6fc3, resolution 1.75Å

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