4qi6: Difference between revisions
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qi6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qi6 OCA], [http://pdbe.org/4qi6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qi6 RCSB], [http://www.ebi.ac.uk/pdbsum/4qi6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qi6 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qi6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qi6 OCA], [http://pdbe.org/4qi6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qi6 RCSB], [http://www.ebi.ac.uk/pdbsum/4qi6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qi6 ProSAT]</span></td></tr> | ||
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== Publication Abstract from PubMed == | |||
A new paradigm for cellulose depolymerization by fungi focuses on an oxidative mechanism involving cellobiose dehydrogenases (CDH) and copper-dependent lytic polysaccharide monooxygenases (LPMO); however, mechanistic studies have been hampered by the lack of structural information regarding CDH. CDH contains a haem-binding cytochrome (CYT) connected via a flexible linker to a flavin-dependent dehydrogenase (DH). Electrons are generated from cellobiose oxidation catalysed by DH and shuttled via CYT to LPMO. Here we present structural analyses that provide a comprehensive picture of CDH conformers, which govern the electron transfer between redox centres. Using structure-based site-directed mutagenesis, rapid kinetics analysis and molecular docking, we demonstrate that flavin-to-haem interdomain electron transfer (IET) is enabled by a haem propionate group and that rapid IET requires a closed CDH state in which the propionate is tightly enfolded by DH. Following haem reduction, CYT reduces LPMO to initiate oxygen activation at the copper centre and subsequent cellulose depolymerization. | |||
Structural basis for cellobiose dehydrogenase action during oxidative cellulose degradation.,Tan TC, Kracher D, Gandini R, Sygmund C, Kittl R, Haltrich D, Hallberg BM, Ludwig R, Divne C Nat Commun. 2015 Jul 7;6:7542. doi: 10.1038/ncomms8542. PMID:26151670<ref>PMID:26151670</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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== References == | |||
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