2eig: Difference between revisions

Revision as of 02:35, 4 May 2008

Lotus tetragonolobus seed lectin (Isoform)

OverviewOverview

Lotus tetragonolobus lectin (LTA) is a fucose-specific legume lectin. Although several studies report a diverse combination of biological activities for LTA, little is known about the mechanisms involved in l-fucosyl oligosaccharide recognition. The crystal structure of LTA at 2.0A resolution reveals a different legume lectin tetramer. Its structure consists of a homotetramer composed of two back-to-back GS4-like dimers arranged in a new mode, resulting in a novel tetramer. The LTA N-linked carbohydrate at Asn4 and the unusual LTA dimer-dimer interaction are related to its particular mode of tetramerization. In addition, we used small angle X-ray scattering to investigate the quaternary structure of LTA in solution and to compare it to the crystalline structure. Although the crystal structure of LTA has revealed a conserved metal-binding site, its l-fucose-binding site presents some punctual differences. Our investigation of the new tetramer of LTA and its fucose-binding site is essential for further studies related to cross-linking between LTA and complex divalent l-fucosyl carbohydrates.

About this StructureAbout this Structure

2EIG is a Single protein structure of sequence from Lotus tetragonolobus. Full crystallographic information is available from OCA.

ReferenceReference

Identification of a new quaternary association for legume lectins., Moreno FB, de Oliveira TM, Martil DE, Vicoti MM, Bezerra GA, Abrego JR, Cavada BS, Filgueira de Azevedo W Jr, J Struct Biol. 2008 Feb;161(2):133-43. Epub 2007 Oct 15. PMID:18068379 Page seeded by OCA on Sun May 4 02:35:54 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2eig.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2eig |SIZE=350|CAPTION= <scene name='initialview01'>2eig</scene>, resolution 2.00&Aring;
+The line below this paragraph, containing "STRUCTURE_2eig", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2eig|  PDB=2eig  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eig OCA], [http://www.ebi.ac.uk/pdbsum/2eig PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2eig RCSB]</span>
-}}
 '''Lotus tetragonolobus seed lectin (Isoform)'''
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 [[Category: Oliveira, T M.de.]]
 [[Category: Vicoti, M M.]]
-[[Category: l-fucosyl]]
+[[Category: L-fucosyl]]
-[[Category: lotus tetragonolobus]]
+[[Category: Lotus tetragonolobus]]
-[[Category: n-acetyl-d-glucosamine]]
+[[Category: N-acetyl-d-glucosamine]]
-[[Category: sugar binding protein]]
+[[Category: Sugar binding protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 02:35:54 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:49:35 2008''