2efw: Difference between revisions

Revision as of 02:29, 4 May 2008

Crystal structure of the RTP:nRB complex from Bacillus subtilis

OverviewOverview

In Bacillus subtilis, the termination of DNA replication via polar fork arrest is effected by a specific protein:DNA complex formed between the replication terminator protein (RTP) and DNA terminator sites. We report the crystal structure of a replication terminator protein homologue (RTP.C110S) of B. subtilis in complex with the high affinity component of one of its cognate DNA termination sites, known as the TerI B-site, refined at 2.5 A resolution. The 21 bp RTP:DNA complex displays marked structural asymmetry in both the homodimeric protein and the DNA. This is in contrast to the previously reported complex formed with a symmetrical TerI B-site homologue. The induced asymmetry is consistent with the complex's solution properties as determined using NMR spectroscopy. Concomitant with this asymmetry is variation in the protein:DNA binding pattern for each of the subunits of the RTP homodimer. It is proposed that the asymmetric "wing" positions, as well as other asymmetrical features of the RTP:DNA complex, are critical for the cooperative binding that underlies the mechanism of polar fork arrest at the complete terminator site.

About this StructureAbout this Structure

2EFW is a Protein complex structure of sequences from Bacillus subtilis. Full crystallographic information is available from OCA.

ReferenceReference

An asymmetric structure of the Bacillus subtilis replication terminator protein in complex with DNA., Vivian JP, Porter CJ, Wilce JA, Wilce MC, J Mol Biol. 2007 Jul 13;370(3):481-91. Epub 2007 Mar 2. PMID:17521668 Page seeded by OCA on Sun May 4 02:29:20 2008

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OCA

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 [[Image:2efw.jpg|left|200px]]
- {{Structure
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-|GENE= rtp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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-|RELATEDENTRY=[[1bm9|1bm9]], [[1j0r|1J0R]], [[1f4k|1F4K]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2efw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2efw OCA], [http://www.ebi.ac.uk/pdbsum/2efw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2efw RCSB]</span>
-}}
 '''Crystal structure of the RTP:nRB complex from Bacillus subtilis'''
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 [[Category: Wilce, J A.]]
 [[Category: Wilce, M C.J.]]
-[[Category: winged -helix protein]]
+[[Category: Winged -helix protein]]
-[[Category: dna replication termination]]
+[[Category: Dna replication termination]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
-[[Category: replication fork arrest]]
+[[Category: Replication fork arrest]]
-[[Category: replication/dna complex]]
+[[Category: Replication/dna complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 02:29:20 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:48:31 2008''