1ogv: Difference between revisions
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
==Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides== | ==Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides== | ||
<StructureSection load='1ogv' size='340' side='right' caption='[[1ogv]], [[Resolution|resolution]] 2.35Å' scene=''> | <StructureSection load='1ogv' size='340' side='right'caption='[[1ogv]], [[Resolution|resolution]] 2.35Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1ogv]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OGV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OGV FirstGlance]. <br> | <table><tr><td colspan='2'>[[1ogv]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OGV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OGV FirstGlance]. <br> | ||
Line 33: | Line 33: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Rhodobacter sphaeroides]] | [[Category: Rhodobacter sphaeroides]] | ||
[[Category: Andreasson, L E]] | [[Category: Andreasson, L E]] |
Revision as of 21:26, 20 November 2019
Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroidesLipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides
Structural highlights
Function[RCEH_RHOSH] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [RCEM_RHOSH] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [RCEL_RHOSH] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. Evolutionary Conservation![]() Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedWell-ordered crystals of the bacterial photosynthetic reaction centre from Rhodobacter sphaeroides were grown from a lipidic cubic phase. Here, we report the type I crystal packing that results from this crystallisation medium, for which 3D crystals grow as stacked 2D crystals, and the reaction centre X-ray structure is refined to 2.35A resolution. In this crystal form, the location of the membrane bilayer could be assigned with confidence. A cardiolipin-binding site is found at the protein-protein interface within the membrane-spanning region, shedding light on the formation of crystal contacts within the membrane. A chloride-binding site was identified in the membrane-spanning region, which suggests a putative site for interaction with the light-harvesting complex I, the cytochrome bc(1) complex or PufX. Comparisons with the X-ray structures of this reaction centre deriving from detergent-based crystals are drawn, indicating that a slight compression occurs in this lipid-rich environment. Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2.35A resolution.,Katona G, Andreasson U, Landau EM, Andreasson LE, Neutze R J Mol Biol. 2003 Aug 15;331(3):681-92. PMID:12899837[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
|