2e09: Difference between revisions

Revision as of 01:42, 4 May 2008

Structure of mutant tryptophan synthase alpha-subunit (E74A) from a hyperthermophile, Pyrococcus furiosus

OverviewOverview

The structure of the tryptophan synthase alpha-subunit from Pyrococcus furiosus was determined by x-ray analysis at 2.0-A resolution, and its stability was examined by differential scanning calorimetry. Although the structure of the tryptophan synthase alpha(2)beta(2) complex from Salmonella typhimurium has been already determined, this is the first report of the structure of the alpha-subunit alone. The alpha-subunit from P. furiosus (Pf-alpha-subunit) lacked 12 and 6 residues at the N and C termini, respectively, and one residue each in two loop regions as compared with that from S. typhimurium (St-alpha-subunit), resulting in the absence of an N-terminal helix and the shortening of a C-terminal helix. The structure of the Pf-alpha-subunit was essentially similar to that of the St-alpha-subunit in the alpha(2)beta(2) complex. The differences between both structures were discussed in connection with the higher stability of the Pf-alpha-subunit and the complex formation of the alpha- and beta-subunits. Calorimetric results indicated that the Pf-alpha-subunit has extremely high thermostability and that its higher stability is caused by an entropic effect. On the basis of structural information of both proteins, we analyzed the contributions of each stabilization factor and could conclude that hydrophobic interactions in the protein interior do not contribute to the higher stability of the Pf-alpha-subunit. Rather, the increase in ion pairs, decrease in cavity volume, and entropic effects due to shortening of the polypeptide chain play important roles in extremely high stability in Pf-alpha-subunit.

About this StructureAbout this Structure

2E09 is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.

ReferenceReference

Entropic stabilization of the tryptophan synthase alpha-subunit from a hyperthermophile, Pyrococcus furiosus. X-ray analysis and calorimetry., Yamagata Y, Ogasahara K, Hioki Y, Lee SJ, Nakagawa A, Nakamura H, Ishida M, Kuramitsu S, Yutani K, J Biol Chem. 2001 Apr 6;276(14):11062-71. Epub 2000 Dec 15. PMID:11118452 Page seeded by OCA on Sun May 4 01:42:04 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2e09.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2e09 |SIZE=350|CAPTION= <scene name='initialview01'>2e09</scene>, resolution 2.40&Aring;
+The line below this paragraph, containing "STRUCTURE_2e09", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= trpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 Pyrococcus furiosus])
+-->
-|DOMAIN=
+{{STRUCTURE_2e09|  PDB=2e09  |  SCENE=  }}
-|RELATEDENTRY=[[1geq|1GEQ]], [[2dzp|2dzp]], [[2dzs|2dzs]], [[2dzt|2dzt]], [[2dzu|2dzu]], [[2dzv|2dzv]], [[2dzw|2dzw]], [[2dzx|2dzx]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2e09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e09 OCA], [http://www.ebi.ac.uk/pdbsum/2e09 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2e09 RCSB]</span>
-}}
 '''Structure of mutant tryptophan synthase alpha-subunit (E74A) from a hyperthermophile, Pyrococcus furiosus'''
@@ Line 30: / Line 27: @@
 [[Category: Yamagata, Y.]]
 [[Category: Yutani, K.]]
-[[Category: calorimetry]]
+[[Category: Calorimetry]]
-[[Category: hyperthermophile]]
+[[Category: Hyperthermophile]]
-[[Category: lyase]]
+[[Category: Lyase]]
-[[Category: national project on protein structural and functional analyse]]
+[[Category: National project on protein structural and functional analyse]]
-[[Category: nppsfa]]
+[[Category: Nppsfa]]
-[[Category: pyrococcus furiosus]]
+[[Category: Pyrococcus furiosus]]
-[[Category: riken structural genomics/proteomics initiative]]
+[[Category: Riken structural genomics/proteomics initiative]]
-[[Category: rsgi]]
+[[Category: Rsgi]]
-[[Category: stability]]
+[[Category: Stability]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: tryptophan synthase alpha-subunit]]
+[[Category: Tryptophan synthase alpha-subunit]]
-[[Category: x-ray analysis]]
+[[Category: X-ray analysis]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 01:42:04 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:41:50 2008''