2dvg: Difference between revisions

Revision as of 01:27, 4 May 2008

Crystal structure of peanut lectin GAL-ALPHA-1,6-GLC complex

OverviewOverview

Crystal structures of peanut lectin complexed with Galbeta1-3Gal, methyl-T-antigen, Galbeta1-6GalNAc, Galalpha1-3Gal and Galalpha1-6Glc and that of a crystal grown in the presence of Galalpha1-3Galbeta1-4Gal have been determined using data collected at 100 K. The use of water bridges as a strategy for generating carbohydrate specificity was previously deduced from the complexes of the lectin with lactose (Galbeta1-4Glc) and T-antigen (Galbeta1-3GalNAc). This has been confirmed by the analysis of the complexes with Galbeta1-3Gal and methyl-T-antigen (Galbeta1-3GalNAc-alpha-OMe). A detailed analysis of lectin-sugar interactions in the complexes shows that they are more extensive when the beta-anomer is involved in the linkage. As expected, the second sugar residue is ill-defined when the linkage is 1-->6. There are more than two dozen water molecules which occur in the hydration shells of all structures determined at resolutions better than 2.5 A. Most of them are involved in stabilizing the structure, particularly loops. Water molecules involved in lectin-sugar interactions are also substantially conserved. The lectin molecule is fairly rigid and does not appear to be affected by changes in temperature.

About this StructureAbout this Structure

2DVG is a Single protein structure of sequence from Arachis hypogaea. Full crystallographic information is available from OCA.

ReferenceReference

Structural studies on peanut lectin complexed with disaccharides involving different linkages: further insights into the structure and interactions of the lectin., Natchiar SK, Srinivas O, Mitra N, Surolia A, Jayaraman N, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1413-21. Epub 2006, Oct 18. PMID:17057347 Page seeded by OCA on Sun May 4 01:27:56 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2dvg.gif|left|200px]]
- {{Structure
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-|RELATEDENTRY=[[2pel|2PEL]], [[2tep|2TEP]], [[1v6i|1V6I]], [[1v6j|1V6J]], [[1v6k|1V6K]], [[2dh1|2DH1]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dvg OCA], [http://www.ebi.ac.uk/pdbsum/2dvg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dvg RCSB]</span>
-}}
 '''Crystal structure of peanut lectin GAL-ALPHA-1,6-GLC complex'''
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 [[Category: Surolia, A.]]
 [[Category: Vijayan, M.]]
-[[Category: agglutinin]]
+[[Category: Agglutinin]]
-[[Category: carbohydrate specificity]]
+[[Category: Carbohydrate specificity]]
-[[Category: legume lectin]]
+[[Category: Legume lectin]]
-[[Category: open quaternary structure]]
+[[Category: Open quaternary structure]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 01:27:56 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:40:04 2008''