5oqa: Difference between revisions
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==XFEL structure of the off state of a reversibly photoswitching fluorescent protein determined using the GDVN injection method== | ==XFEL structure of the off state of a reversibly photoswitching fluorescent protein determined using the GDVN injection method== | ||
<StructureSection load='5oqa' size='340' side='right' caption='[[5oqa]], [[Resolution|resolution]] 1.53Å' scene=''> | <StructureSection load='5oqa' size='340' side='right'caption='[[5oqa]], [[Resolution|resolution]] 1.53Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5oqa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lobhe Lobhe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OQA FirstGlance]. <br> | <table><tr><td colspan='2'>[[5oqa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lobhe Lobhe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OQA FirstGlance]. <br> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Lobhe]] | [[Category: Lobhe]] | ||
[[Category: Cordon-Preciado, V]] | [[Category: Cordon-Preciado, V]] | ||
Revision as of 09:57, 16 October 2019
XFEL structure of the off state of a reversibly photoswitching fluorescent protein determined using the GDVN injection methodXFEL structure of the off state of a reversibly photoswitching fluorescent protein determined using the GDVN injection method
Structural highlights
Publication Abstract from PubMedThe photochromic fluorescent protein Skylan-NS (Nonlinear Structured illumination variant mEos3.1H62L) is a reversibly photoswitchable fluorescent protein which has an unilluminated/ground state with an anionic and cis chromophore conformation and high fluorescence quantum yield. Photo-conversion with illumination at 515 nm generates a meta-stable intermediate with neutral trans-chromophore structure that has a 4 h lifetime. We present X-ray crystal structures of the cis (on) state at 1.9 Angstrom resolution and the trans (off) state at a limiting resolution of 1.55 Angstrom from serial femtosecond crystallography experiments conducted at SPring-8 Angstrom Compact Free Electron Laser (SACLA) at 7.0 keV and 10.5 keV, and at Linac Coherent Light Source (LCLS) at 9.5 keV. We present a comparison of the data reduction and structure determination statistics for the two facilities which differ in flux, beam characteristics and detector technologies. Furthermore, a comparison of droplet on demand, grease injection and Gas Dynamic Virtual Nozzle (GDVN) injection shows no significant differences in limiting resolution. The photoconversion of the on- to the off-state includes both internal and surface exposed protein structural changes, occurring in regions that lack crystal contacts in the orthorhombic crystal form. X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature.,Hutchison CDM, Cordon-Preciado V, Morgan RML, Nakane T, Ferreira J, Dorlhiac G, Sanchez-Gonzalez A, Johnson AS, Fitzpatrick A, Fare C, Marangos JP, Yoon CH, Hunter MS, DePonte DP, Boutet S, Owada S, Tanaka R, Tono K, Iwata S, van Thor JJ Int J Mol Sci. 2017 Sep 7;18(9). pii: ijms18091918. doi: 10.3390/ijms18091918. PMID:28880248[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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