2dhq: Difference between revisions

Revision as of 00:26, 4 May 2008

3-DEHYDROQUINATE DEHYDRATASE FROM MYCOBACTERIUM TUBERCULOSIS

OverviewOverview

The structures of enzymes catalyzing the reactions in central metabolic pathways are generally well conserved as are their catalytic mechanisms. The two types of 3-dehydroquinate dehydratase (DHQase) are therefore most unusual since they are unrelated at the sequence level and they utilize completely different mechanisms to catalyze the same overall reaction. The type I enzymes catalyze a cis-dehydration of 3-dehydroquinate via a covalent imine intermediate, while the type II enzymes catalyze a trans-dehydration via an enolate intermediate. Here we report the three-dimensional structures of a representative member of each type of biosynthetic DHQase. Both enzymes function as part of the shikimate pathway, which is essential in microorganisms and plants for the biosynthesis of aromatic compounds including folate, ubiquinone and the aromatic amino acids. An explanation for the presence of two different enzymes catalyzing the same reaction is presented. The absence of the shikimate pathway in animals makes it an attractive target for antimicrobial agents. The availability of these two structures opens the way for the design of highly specific enzyme inhibitors with potential importance as selective therapeutic agents.

About this StructureAbout this Structure

2DHQ is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

The two types of 3-dehydroquinase have distinct structures but catalyze the same overall reaction., Gourley DG, Shrive AK, Polikarpov I, Krell T, Coggins JR, Hawkins AR, Isaacs NW, Sawyer L, Nat Struct Biol. 1999 Jun;6(6):521-5. PMID:10360352 Page seeded by OCA on Sun May 4 00:26:51 2008

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OCA

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 [[Image:2dhq.jpg|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-dehydroquinate_dehydratase 3-dehydroquinate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.10 4.2.1.10] </span>
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dhq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dhq OCA], [http://www.ebi.ac.uk/pdbsum/2dhq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dhq RCSB]</span>
-}}
 '''3-DEHYDROQUINATE DEHYDRATASE FROM MYCOBACTERIUM TUBERCULOSIS'''
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 [[Category: Hawkins, A R.]]
 [[Category: Isaacs, N W.]]
-[[Category: alpha/beta protein]]
+[[Category: Alpha/beta protein]]
-[[Category: dehydratase]]
+[[Category: Dehydratase]]
-[[Category: shikimate pathway]]
+[[Category: Shikimate pathway]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 00:26:51 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:34:51 2008''