2d11: Difference between revisions

Revision as of 23:29, 3 May 2008

Crystal structure of the Radixin FERM domain complexed with the NHERF-2 C-terminal tail peptide

OverviewOverview

The Na+/H+ exchanger regulatory factor (NHERF) is a key adaptor protein involved in the anchoring of ion channels and receptors to the actin cytoskeleton through binding to ERM (ezrin/radixin/moesin) proteins. NHERF binds the FERM domain of ERM proteins, although NHERF has no signature Motif-1 sequence for FERM binding found in adhesion molecules. The crystal structures of the radixin FERM domain complexed with the NHERF-1 and NHERF-2 C-terminal peptides revealed a peptide binding site of the FERM domain specific for the 13 residue motif MDWxxxxx(L/I)Fxx(L/F) (Motif-2), which is distinct from Motif-1. This Motif-2 forms an amphipathic alpha helix for hydrophobic docking to subdomain C of the FERM domain. This docking causes induced-fit conformational changes in subdomain C and affects binding to adhesion molecule peptides, while the two binding sites are not overlapped. Our studies provide structural paradigms for versatile ERM linkages between membrane proteins and the cytoskeleton.

About this StructureAbout this Structure

2D11 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for NHERF recognition by ERM proteins., Terawaki S, Maesaki R, Hakoshima T, Structure. 2006 Apr;14(4):777-89. PMID:16615918 Page seeded by OCA on Sat May 3 23:29:43 2008

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OCA

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 '''Crystal structure of the Radixin FERM domain complexed with the NHERF-2 C-terminal tail peptide'''
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 [[Category: Maesaki, R.]]
 [[Category: Terawaki, S.]]
-[[Category: protein-peptide complex]]
+[[Category: Protein-peptide complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 23:29:43 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:28:52 2008''