1u9b: Difference between revisions
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[[Image:1u9b.gif|left|200px]]<br /> | [[Image:1u9b.gif|left|200px]]<br /><applet load="1u9b" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1u9b" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1u9b, resolution 2.0Å" /> | caption="1u9b, resolution 2.0Å" /> | ||
'''MURINE/HUMAN UBIQUITIN-CONJUGATING ENZYME UBC9'''<br /> | '''MURINE/HUMAN UBIQUITIN-CONJUGATING ENZYME UBC9'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1U9B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] | 1U9B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Known structural/functional Site: <scene name='pdbsite=C93:Ubiquitin Is Linked To Ubc9 Via CYS 93'>C93</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U9B OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: ubiquitin-directed proteolysis]] | [[Category: ubiquitin-directed proteolysis]] | ||
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Revision as of 18:53, 18 December 2007
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MURINE/HUMAN UBIQUITIN-CONJUGATING ENZYME UBC9
OverviewOverview
Murine/human ubiquitin-conjugating enzyme Ubc9 is a functional homolog of, Saccharomyces cerevisiae Ubc9 that is essential for the viability of yeast, cells with a specific role in the G2-M transition of the cell cycle. The, structure of recombinant mammalian Ubc9 has been determined from two, crystal forms at 2.0 A resolution. Like Arabidopsis thaliana Ubc1 and S., cerevisiae Ubc4, murine/human Ubc9 was crystallized as a monomer, suggesting that previously reported hetero- and homo-interactions among, Ubcs may be relatively weak or indirect. Compared with the known crystal, structures of Ubc1 and Ubc4, which regulate different cellular processes, Ubc9 has a 5-residue insertion that forms a very exposed tight, beta-hairpin and a 2-residue insertion that forms a bulge in a loop close, to the active site. Mammalian Ubc9 also possesses a distinct electrostatic, potential distribution that may provide possible clues to its remarkable, ability to interact with other proteins. The 2-residue insertion and other, sequence and structural heterogeneity observed at the catalytic site, suggest that different Ubcs may utilize catalytic mechanisms of varying, efficiency and substrate specificity.
About this StructureAbout this Structure
1U9B is a Single protein structure of sequence from Mus musculus. Active as Ubiquitin--protein ligase, with EC number 6.3.2.19 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of murine/human Ubc9 provides insight into the variability of the ubiquitin-conjugating system., Tong H, Hateboer G, Perrakis A, Bernards R, Sixma TK, J Biol Chem. 1997 Aug 22;272(34):21381-7. PMID:9261152
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